A conserved repetitive DNA element located in the centromeres of cereal chromosomes
| Autor: | Shuhei Nasuda, Rod A. Wing, Jiming Jiang, Christopher W. Scherrer, Sung Sick Woo, Bikram S. Gill, Fenggao Dong, David C. Ward |
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| Rok vydání: | 1996 |
| Předmět: |
Centromere
Molecular Sequence Data Biology DNA sequencing chemistry.chemical_compound Schizosaccharomyces medicine Homologous chromosome Animals Humans Repeated sequence Conserved Sequence In Situ Hybridization Fluorescence Gene Library Repetitive Sequences Nucleic Acid Genetics B chromosome Multidisciplinary Base Sequence medicine.diagnostic_test Chromosome Mapping food and beverages Plants Biological Sciences Drosophila melanogaster chemistry Eukaryotic chromosome fine structure Edible Grain DNA Fluorescence in situ hybridization |
| Zdroj: | Proceedings of the National Academy of Sciences. 93:14210-14213 |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.93.24.14210 |
| Popis: | Repetitive DNA sequences have been demonstrated to play an important role for centromere function of eukaryotic chromosomes, including those from fission yeast, Drosophila melanogaster, and humans. Here we report on the isolation of a repetitive DNA element located in the centromeric regions of cereal chromosomes. A 745-bp repetitive DNA clone, pSau3A9, was isolated from sorghum ( Sorghum bicolor ). This DNA element is located in the centromeric regions of all sorghum chromosomes, as demonstrated by fluorescence in situ hybridization. Repetitive DNA sequences homologous to pSau3A9 also are present in the centromeric regions of chromosomes from other cereal species, including rice, maize, wheat, barley, rye, and oats. Probe pSau3A9 also hybridized to the centromeric region of B chromosomes from rye and maize. The repetitive nature and its conservation in distantly related plant species indicate that the pSau3A9 family may be associated with centromere function of cereal chromosomes. The absence of DNA sequences homologous to pSau3A9 in dicot species suggests a faster divergence of centromere-related sequences compared with the telomere-related sequences in plants. |
| Databáze: | OpenAIRE |
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