Differential downstream effects of CD40 ligation mediated by membrane or soluble CD40L and agonistic Ab: a study on purified human B cells
| Autor: | L. Beniguel, C. Genin, Yolande Richard, Patricia Chavarin, Fabrice Cognasse, O. Sabido, S. Acquart, Olivier Garraud |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Immunology
Cell Antigens CD19 Palatine Tonsil Enzyme-Linked Immunosorbent Assay Transfection CD19 03 medical and health sciences Mice 0302 clinical medicine medicine Immunology and Allergy Animals Humans CD40 Antigens B cell Cells Cultured Pharmacology CD86 B-Lymphocytes CD40 biology Chemistry Cell Membrane Antibodies Monoclonal hemic and immune systems Cell Differentiation Fibroblasts Flow Cytometry Molecular biology In vitro Immunoglobulin A Immunoglobulin Fc Fragments medicine.anatomical_structure Immunoglobulin M Polyclonal antibodies 030220 oncology & carcinogenesis Immunoglobulin G biology.protein Indicators and Reagents Immunoglobulin Heavy Chains CD80 030215 immunology |
| Zdroj: | International journal of immunopathology and pharmacology. 18(1) |
| ISSN: | 0394-6320 |
| Popis: | With the addition of various cytokines, the CD40-CD40 ligand (CD40L) system can act as a T-helper cell surrogate to permit B lymphocytes to produce large amounts of polyclonal Ig. In the present study, we tested six CD40-CD40L stimulation models: (i, ii) soluble agonistic 89 and G28.5 mAbs; (iii, iv) ‘89’ and ‘G28.5’ bound via their Fc fragments on CDw32-transfected mouse fibroblasts; (v) purified, soluble, trimeric human CD40L molecules (sCD40L); and (vi) human CD40L expressed by a CD40L-transfected mouse fibroblastic cell line (LCD40L). Target B cells consisted of purified blood and tonsillar CD19+ lymphocytes cultured in the presence of CD40 stimuli and IL-2 and IL-10, added at the onset of each B cell culture. A) There was differential expression of CD69, CD80 and CD86 exposure to sCD40L and LCD40L was ensued by the strongest % MFI changes over control. B) In blood B cells, mAbs and sCD40L induced IgA, IgM and IgG production almost equally well; LCD40L proved less efficient. In contrast, in tonsil B cells, LCD40L induced significantly more IgA, IgG 1, IgG3and IgM production than other signals. Using certain CD40/CD40L stimuli to model in vitro Ig production, a system used regularly in many laboratories, may affect the interpretation based on the cell type and on the CD40/CD40L system used. |
| Databáze: | OpenAIRE |
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