Extensible multiplex real-time PCR for rapid bacterial identification with carbon nanotube composite microparticles
| Autor: | Seungwon Jung, Sang Kyung Kim, Jungmin Kim, Junsun Kim, Sang Hwa Yang |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Biomedical Engineering Biophysics Biosensing Techniques 02 engineering and technology Carbon nanotube Biology Real-Time Polymerase Chain Reaction medicine.disease_cause law.invention 03 medical and health sciences law Multiplex polymerase chain reaction Electrochemistry medicine Multiplex Polymerase chain reaction DNA Primers Bacteria Nanotubes Carbon Pathogenic bacteria General Medicine 021001 nanoscience & nanotechnology biology.organism_classification Molecular biology 030104 developmental biology Real-time polymerase chain reaction Primer (molecular biology) 0210 nano-technology Multiplex Polymerase Chain Reaction Biotechnology |
| Zdroj: | Biosensors and Bioelectronics. 94:256-262 |
| ISSN: | 0956-5663 |
| Popis: | The early diagnosis of pathogenic bacteria is significant for bacterial identification and antibiotic resistance. Implementing rapid, sensitive, and specific detection, molecular diagnosis has been considered complementary to the conventional bacterial culture. Composite microparticles of a primer-immobilized network (cPIN) are developed for multiplex detection of pathogenic bacteria with real-time polymerase chain reaction (qPCR). A pair of specific primers are incorporated and stably conserved in a cPIN particle. One primer is crosslinked to the polymer network, and the other is bound to carbon nanotubes (CNTs) in the particle. At the initiation of qPCR, the latter primer is released from the CNTs and participates in the amplification. The amplification efficiency of this cPIN qPCR is estimated at more than 90% with suppressed non-specific signals from complex samples. In multiplexing, four infective pathogens are successfully discriminated using this cPIN qPCR. Multiplex qPCR conforms with the corresponding singleplex assays, proving independent amplification in each particle. Four bacterial targets from clinical samples are differentially analyzed in 30min of a single qPCR trial with multiple cPIN particles. |
| Databáze: | OpenAIRE |
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