High proportion of PD-1-expressing CD4 + T cells in adipose tissue constitutes an immunomodulatory microenvironment that may support HIV persistence
| Autor: | Guillaume Pourcher, Abderaouf Damouche, Benoit Favier, Jean Jacques Lataillade, Bruno Vaslin, Valérie Pourcher, Christine Bourgeois, Elodie Busson, Olivier Lambotte, Mélanie Le Van, Stéphane Benoist, Thierry Lazure, Julien Adam, Michaela Müller-Trutwin |
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| Přispěvatelé: | Martin, Marie, Immunologie des Maladies Virales et Autoimmunes (IMVA - U1184), Université Paris-Sud - Paris 11 (UP11)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de la Santé et de la Recherche Médicale (INSERM), Département de Pathologies digestives [Institut Mutualiste Montsouris], Institut Mutualiste de Montsouris (IMM)-Centre de prise en charge de la maladie obésité [Institut Mutualiste Montsouris], Institut Mutualiste de Montsouris (IMM), Service de Maladies Infectieuses et Tropicales [CHU Pitié-Salpêtrière], CHU Pitié-Salpêtrière [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Service de Chirurgie générale et digestive [CHU Le Kremlin-Bicêtre], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-AP-HP Hôpital Bicêtre (Le Kremlin-Bicêtre), Hôpital d'instruction des Armées Percy, Service de Santé des Armées, AP-HP Hôpital Bicêtre (Le Kremlin-Bicêtre), Plateforme d’évaluation préclinique (PFEP), Analyse moléculaire, modélisation et imagerie de la maladie cancéreuse (AMMICa), Université Paris-Sud - Paris 11 (UP11)-Institut Gustave Roussy (IGR)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Paris-Sud - Paris 11 (UP11)-Institut Gustave Roussy (IGR)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), HIV, Inflammation et persistance - HIV, Inflammation and Persistence, Institut Pasteur [Paris] (IP), Service de Médecine Interne et Immunologie clinique [AP-HP Hôpital Bicêtre], Hôpital Bicêtre, Régulation de la réponse immune, infection VIH-1 et autoimmunité, Université Paris-Sud - Paris 11 (UP11)-Institut National de la Santé et de la Recherche Médicale (INSERM), Agence Nationale de Recherches sur le Sida et les Hepatites Virales. Grant Number: NSA17004LRASidaction. Grant Numbers: 10613, 16‐2‐1‐FJC‐10573, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Service des maladies infectieuses et tropicales [CHU Pitié-Salpêtrière], HIV, Inflammation et persistance, Institut Pasteur [Paris], Centre National de la Recherche Scientifique (CNRS)-Université Paris-Sud - Paris 11 (UP11)-Institut Gustave Roussy (IGR)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Paris-Sud - Paris 11 (UP11)-Institut Gustave Roussy (IGR)-Institut National de la Santé et de la Recherche Médicale (INSERM) |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Cell Human immunodeficiency virus (HIV) Adipose tissue HIV persistence MESH: Flow Cytometry MESH: CD4-Positive T-Lymphocytes/virology medicine.disease_cause Persistence (computer science) 0302 clinical medicine MESH: Aged 80 and over PD-1 Immunology and Allergy [SDV.MP.VIR] Life Sciences [q-bio]/Microbiology and Parasitology/Virology MESH: Aged MESH: Disease Reservoirs/virology MESH: Middle Aged MESH: HIV Infections/virology 3. Good health medicine.anatomical_structure MESH: Host-Pathogen Interactions/immunology 030220 oncology & carcinogenesis MESH: Programmed Cell Death 1 Receptor/immunology [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology [SDV.IMM]Life Sciences [q-bio]/Immunology [SDV.IMM] Life Sciences [q-bio]/Immunology Adipose tissue macrophages T cell Immunology Biology MESH: Antiviral Agents/therapeutic use MESH: CD4-Positive T-Lymphocytes/immunology 03 medical and health sciences Immune system medicine MESH: Adipose Tissue/virology MESH: Programmed Cell Death 1 Receptor/metabolism MESH: HIV-1/immunology MESH: Humans MESH: Adipose Tissue/immunology MESH: CD4-Positive T-Lymphocytes/metabolism MESH: HIV Infections/metabolism HIV MESH: HIV Infections/immunology MESH: Adult CD4 T cell MESH: Male 030104 developmental biology Fat MESH: Adipose Tissue/metabolism MESH: Cellular Microenvironment/immunology MESH: Female MESH: HIV-1/drug effects CD8 |
| Zdroj: | European Journal of Immunology European Journal of Immunology, 2017, 47 (12), pp.2113-2123. ⟨10.1002/eji.201747060⟩ European Journal of Immunology, Wiley-VCH Verlag, 2017, 47 (12), pp.2113-2123. ⟨10.1002/eji.201747060⟩ |
| ISSN: | 0014-2980 1521-4141 |
| DOI: | 10.1002/eji.201747060 |
| Popis: | We and others have demonstrated that adipose tissue is a reservoir for HIV. Evaluation of the mechanisms responsible for viral persistence may lead to ways of reducing these reservoirs. Here, we evaluated the immune characteristics of adipose tissue in HIV-infected patients receiving antiretroviral therapy (ART) and in non-HIV-infected patients. We notably sought to determine whether adipose tissue's intrinsic properties and/or HIV induced alteration of the tissue environment may favour viral persistence. ART-controlled HIV infection was associated with a difference in the CD4/CD8 T-cell ratio and an elevated proportion of Treg cells in subcutaneous adipose tissue. No changes in Th1, Th2 and Th17 cell proportions or activation markers expression on T cell (Ki-67, HLA-DR) could be detected, and the percentage of CD69-expressing resident memory CD4+ T cells was not affected. Overall, our results indicate that adipose-tissue-resident CD4+ T cells are not extensively activated during HIV infection. PD-1 was expressed by a high proportion of tissue-resident memory CD4+ T cells in both HIV-infected patients and non-HIV-infected patients. Our findings suggest that adipose tissue's intrinsic immunomodulatory properties may limit immune activation and thus may strongly contribute to viral persistence. This article is protected by copyright. All rights reserved |
| Databáze: | OpenAIRE |
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