Differential expression of integrin alpha v and beta 3 in serosal tissue of human intraperitoneal organs and adhesion
| Autor: | Lena Holmdahl, Michael P. Diamond, Nasser Chegini, Chunfeng Ma, R. Stan Williams, Kristina Kotseos, Keven Skinner |
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| Rok vydání: | 2001 |
| Předmět: |
Adult
Male Pathology medicine.medical_specialty Transcription Genetic Integrin Uterus Adhesion (medicine) Ovary Tissue Adhesions Platelet Membrane Glycoproteins Biology Peritoneum Antigens CD Gene expression Intestine Small medicine Humans RNA Messenger Fallopian Tubes Aged Skin Aged 80 and over Reverse Transcriptase Polymerase Chain Reaction Integrin beta3 Obstetrics and Gynecology Integrin alphaV Middle Aged medicine.disease Reverse transcription polymerase chain reaction Postmenopause medicine.anatomical_structure Reproductive Medicine Premenopause biology.protein Female Subcutaneous tissue |
| Zdroj: | Fertility and sterility. 75(4) |
| ISSN: | 0015-0282 |
| Popis: | Objective: To assess the expression of integrin αv and β3 in the serosal tissue of intraperitoneal organs and adhesions in persons with and without adhesions. Design: Prospective study. Setting: Academic research centers. Patient(s): Fifty-seven patients undergoing abdominal or pelvic surgery. Main Outcome Measure(s): Integrin αv and β3 messenger RNA (mRNA) expression was evaluated by quantitative reverse transcription polymerase chain reaction. Result(s): The serosal tissue of the parietal peritoneum, uterus, fallopian tubes, ovary, and the large and small bowel, as well as peritoneal adhesions, skin, fascia, subcutaneous tissue, and omentum, expresses integrin αv and β3 mRNA. The level of αv and β3 mRNA expression varied among these tissues; expression of the former substance was highest in uterine serosa and lowest in the skin and small bowel, and expression of the latter substance was highest in the fallopian tubes and skin and lowest in the uterine serosa. Parietal peritoneum and adhesions express equal levels of integrin αv; however, integrin β3 expression was >100-fold lower in adhesions than in peritoneum. The level of integrin β3 expression in omentum, small and large bowels, and subcutaneous tissue was 100-fold to 10,000-fold lower than in other tissues. Conclusion(s): Serosal tissue of peritoneal organs and adhesions express variable levels of integrin αv and β3 mRNA. On the basis of such variation and the knowledge that tissue injury alters local integrin expression, integrins may play a key role in adhesion development, particularly in tissue with higher integrin expression. |
| Databáze: | OpenAIRE |
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