PRIMARY SLX TEST USING REAL-TIME PCR BASED ON HIGH RESOLUTION MELTING (HRM) ON MICROFILARIA EXAMINATION
| Autor: | Cecep Dani Sucipto, Bagus Muhammad Ihsan, Khayan Khayan |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
biology
spesifitas Positive control medicine.disease_cause biology.organism_classification Microbiology Molecular biology Microfilaria QR1-502 Brugia malayi High Resolution Melt law.invention primer slx Wuchereria bancrofti Real-time polymerase chain reaction law mikrofilaria parasitic diseases medicine Medicine Primer (molecular biology) real-time pcr Polymerase chain reaction |
| Zdroj: | Journal of Vocational Health Studies, Vol 5, Iss 1, Pp 26-30 (2021) Journal of Vocational Health Studies; Vol. 5 No. 1 (2021): July 2021 | JOURNAL OF VOCATIONAL HEALTH STUDIES; 26-30 |
| ISSN: | 2580-717X 2580-7161 |
| DOI: | 10.20473/jvhs.v5.i1.2021.26-30 |
| Popis: | Background: Filariasis patients can be a source of transmission if their blood still contains microfilariae. One of the Polymerase Chain Reaction (PCR) methods used is High Resolution Melting (HRM), using primary specificity testing. Purpose: To test the specificity of SLX primer. The samples used for this test were isolates of Salmonella., Klebsiella, Pseudomonas, negative and positive controls for Brugia malayi and Wuchereria bancrofti. Method: The design in this study is a quasi-experiment by testing the specificity of SLX primer using HRMbased real-time PCR based on the Cycle Threshold (CT) value observed through the amplification curve. Result: The real-time PCR results showed that no CT was released in the bacterial samples, and there was a CT value in the positive control. The results of this study indicate that specific SLX primer can be used in identifying microfilariae. Conclusion: SLX primer have a reasonable specificity because they cannot detect the existence of microorganisms in the samples other than microfilariae. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|