Nanopatterned acellular valve conduits drive the commitment of blood-derived multipotent cells
| Autor: | Gino Gerosa, Sandra Schrenk, Paola Aguiari, Rosa Di Liddo, Alessandro Gandaglia, Laura Iop, Maria Teresa Conconi, Silvia Barbon, Amit Mandoli, Pier Paolo Parnigotto, Alessia Tasso, Thomas Bertalot |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Aortic valve Materials science Swine Cellular differentiation Sus scrofa Biophysics Gene Expression Pharmaceutical Science Blood-derived multipotent cells Bioengineering Biomaterials Extracellular matrix 03 medical and health sciences Tissue engineering International Journal of Nanomedicine TriCol decellularization procedure Drug Discovery medicine Animals Self-repopulation potential Heart valve Cells Cultured ECM nanostructure signaling Original Research Heart Valve Prosthesis Implantation Pulmonary Valve Blood Cells Tissue Engineering Tissue Scaffolds Multipotent Stem Cells Drug Discovery3003 Pharmaceutical Science Organic Chemistry Mesenchymal stem cell Endothelial Cells Cell Differentiation Mesenchymal Stem Cells Guided tissue engineering General Medicine Anatomy Extracellular Matrix Nanostructures Cell biology 030104 developmental biology medicine.anatomical_structure Multipotent Stem Cell Aortic Valve Pulmonary valve |
| Zdroj: | International Journal of Nanomedicine |
| ISSN: | 1178-2013 |
| Popis: | Rosa Di Liddo,1,2 Paola Aguiari,3 Silvia Barbon,1,2 Thomas Bertalot,1 Amit Mandoli,1 Alessia Tasso,1 Sandra Schrenk,1 Laura Iop,3 Alessandro Gandaglia,3 Pier Paolo Parnigotto,2 Maria Teresa Conconi,1,2 Gino Gerosa31Department of Pharmaceutical and Pharmacological Sciences, University of Padova, 2Foundation for Biology and Regenerative Medicine, Tissue Engineering and Signaling ONLUS, 3Department of Cardiac, Thoracic and Vascular Sciences, University ofPadova, Padova, Italy Abstract: Considerable progress has been made in recent years toward elucidating the correlation among nanoscale topography, mechanical properties, and biological behavior of cardiac valve substitutes. Porcine TriCol scaffolds are promising valve tissue engineering matrices with demonstrated self-repopulation potentiality. In order to define an in vitro model for investigating the influence of extracellular matrix signaling on the growth pattern of colonizing blood-derived cells, we cultured circulating multipotent cells (CMC) on acellular aortic (AVL) and pulmonary (PVL) valve conduits prepared with TriCol method and under no-flow condition. Isolated by our group from Vietnamese pigs before heart valve prosthetic implantation, porcine CMC revealed high proliferative abilities, three-lineage differentiative potential, and distinct hematopoietic/endothelial and mesenchymal properties. Their interaction with valve extracellular matrix nanostructures boosted differential messenger RNA expression pattern and morphologic features on AVL compared to PVL, while promoting on both matrices the commitment to valvular and endothelial cell-like phenotypes. Based on their origin from peripheral blood, porcine CMC are hypothesized in vivo to exert a pivotal role to homeostatically replenish valve cells and contribute to hetero- or allograft colonization. Furthermore, due to their high responsivity to extracellular matrix nanostructure signaling, porcine CMC could be useful for a preliminary evaluation of heart valve prosthetic functionality. Keywords: blood-derived multipotent cells, self-repopulation potential, guided tissue engineering, TriCol decellularization procedure, ECM nanostructure signaling |
| Databáze: | OpenAIRE |
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