Correlation of genomic detection of feline coronavirus with various diagnostic assays for feline infectious peritonitis
| Autor: | K A Brenneman, L. N. D. Potgieter, R. K. Millsaps, J. Black, Melissa A. Kennedy |
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| Rok vydání: | 1998 |
| Předmět: |
0301 basic medicine
Serotype Feline coronavirus 040301 veterinary sciences 030106 microbiology Virulence Genome Viral Biology medicine.disease_cause Cat Diseases Polymerase Chain Reaction Virus law.invention Feline Infectious Peritonitis 0403 veterinary science 03 medical and health sciences Open Reading Frames law medicine Animals Serologic Tests Fluorescent Antibody Technique Indirect Polymerase chain reaction Coronavirus Electrophoresis Agar Gel General Veterinary 04 agricultural and veterinary sciences Virology Feline infectious peritonitis Cats Coronavirus Infections Nested polymerase chain reaction |
| Zdroj: | Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc. 10(1) |
| ISSN: | 1040-6387 |
| Popis: | Feline infectious peritonitis (FIP) is a fatal disease of domestic and nondomestic felids caused by a feline coronavirus (FCV). It is a significant problem in catteries, multiple cat households, and shelters. 14,18 FIP can manifest as an effusive peritonitis and/or pleuritis, with a relatively short disease course ending in death. A protracted course with granulomatous lesions affecting multiple organs may occur, which also invariably progresses to death. 8,11,12 The FCVs are closely related and include two biotypes: those that are virulent and cause FIP and those that are avirulent. 7 The avirulent group, known as feline enteric coronaviruses (FECV), may be associated with mild enteric disease or subclinical infection in cats. 11,12 FCVs are also classified into serotypes 1 and 2 based on antigenicity. 7,12 Both serotypes contain virulent and avirulent biotypes, and FCVs within a serotype are indistinguishable in the laboratory. FCVs are ubiquitous, especially in environments such as catteries, where large numbers of cats are housed together and where the majority of cats are seropositive. 12 As a result, antemortem diagnosis for FIP is difficult because no detection assay sensitive enough and specific for the virus causing FIP is routinely available. 12 This problem is further compounded because consistent genetic differences between the virulent and avirulent biotypes of FCV have not been identified. 16 An assay has been developed using reverse transcription and a nested polymerase chain reaction (PCR) to detect FCV genomic material in a variety of biologic samples from cats. The genomic region targeted encompasses the 7a and 7b open reading frames (ORFs). This region, specifically that encoding the 7b ORF, may correlate with virulence. |
| Databáze: | OpenAIRE |
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