Epac1 and Epac2 are differentially involved in inflammatory and remodeling processes induced by cigarette smoke
| Autor: | Junjun Cao, Machteld N. Hylkema, Anne Coline Laurent, Anouk Oldenburger, Frank Lezoualc'h, Alan V. Smrcka, H. Maarsingh, Marieke Smit, Herman Meurs, Sophie Bos, Martina Schmidt, Wim Timens |
|---|---|
| Přispěvatelé: | Molecular Pharmacology, Groningen Research Institute for Asthma and COPD (GRIAC), Reproductive Origins of Adult Health and Disease (ROAHD), Guided Treatment in Optimal Selected Cancer Patients (GUTS) |
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
medicine.medical_specialty
GROWTH-FACTOR phospholipase C epsilon medicine.medical_treatment CYCLIC-AMP CRUCIAL ROLE Biology Biochemistry OBSTRUCTIVE PULMONARY-DISEASE Research Communications Cell Line ACTIVATION Immune system In vivo Internal medicine cAMP Smoke Genetics medicine exchange protein COPD Animals Guanine Nucleotide Exchange Factors MACROPHAGES Protein kinase A Molecular Biology PROTEIN-KINASE-A Lung Inflammation Mice Knockout Growth factor Interleukin Mucus Cyclic AMP-Dependent Protein Kinases Fibronectin Mice Inbred C57BL Endocrinology Cytokine biology.protein Airway Remodeling Cytokines Female PHOSPHOLIPASE-C-EPSILON Biotechnology |
| Zdroj: | The FASEB Journal, 28(11), 4617-4628. FEDERATION AMER SOC EXP BIOL |
| ISSN: | 0892-6638 |
| Popis: | Cigarette smoke (CS) induces inflammatory responses characterized by increase of immune cells and cytokine release. Remodeling processes, such as mucus hypersecretion and extracellular matrix protein production, are also directly or indirectly induced by CS. Recently, we showed that activation of the exchange protein directly activated by cAMP (Epac) attenuates CS extract-induced interleukin (IL)-8 release from cultured airway smooth muscle cells. Using an acute, short-term model of CS exposure, we now studied the role of Epac1, Epac2, and the Epac effector phospholipase-Cε (PLCε) in airway inflammation and remodeling in vivo. Compared to wild-type mice exposed to CS, the number of total inflammatory cells, macrophages, and neutrophils and total IL-6 release was lower in Epac2(-/-) mice, which was also the case for neutrophils and IL-6 in PLCε(-/-) mice. Taken together, Epac2, acting partly via PLCε, but not Epac1, enhances CS-induced airway inflammation in vivo. In total lung homogenates of Epac1(-/-) mice, MUC5AC and matrix remodeling parameters (transforming growth factor-β1, collagen I, and fibronectin) were increased at baseline. Our findings suggest that Epac1 primarily is capable of inhibiting remodeling processes, whereas Epac2 primarily increases inflammatory processes in vivo.-Oldenburger, A., Timens, W., Bos, S., Smit, M., Smrcka, A. V., Laurent, A.-C., Cao, J., Hylkema, M., Meurs, H., Maarsingh, H., Lezoualc'h, F., and Schmidt, M. Epac1 and Epac2 are differentially involved in inflammatory and remodeling processes induced by cigarette smoke. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text