A sample-to-answer DNA detection microfluidic system integrating sample pretreatment and smartphone-readable gradient plasmonic photothermal continuous-flow PCR

Autor:	Zengming Zhang, Shuhao Zhao, Lei Jiang, Junjun Wu, Wenhan Zhao, Xiaoniu Guo, Niancai Peng, Fei Hu
Rok vydání:	2022
Předmět:	Microfluidics DNA Viral Electrochemistry Silicone Oils Environmental Chemistry Indicators and Reagents Smartphone Microfluidic Analytical Techniques Polymerase Chain Reaction Biochemistry Spectroscopy Analytical Chemistry
Zdroj:	The Analyst. 147:4876-4887
ISSN:	1364-5528 0003-2654
DOI:	10.1039/d2an00908k
Popis:	As the gold standard for nucleic acid detection, full-process polymerase chain reaction (PCR) analysis often falls into the dilemma of complex workflow, time-consuming, and high equipment costs. Therefore, we designed and optimized a DNA quantification microfluidic system by strategically integrating sample pretreatment and a smartphone-readable gradient plasmonic photothermal (GPPT) continuous-flow PCR (CF-PCR). Through preloading and sequential injection of immiscible extraction reagents, combined with magnetic bead (MB) manipulation, the microfluidic chip successfully purified and concentrated 100 μL of HBV-DNA spiked plasma into a 20-μL purified sample within 14 minutes. With a digital PCR platform, the optimized experiments showed that the DNA extraction efficiency can reach 69% at an immiscible reagent configuration ratio of 10 : 10 : 1 : 12 : 2 (sample : lysis/binding buffer : MB : silicone oil : eluent) and a flow rate of 25 μL min
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dfc351d8342489b2926b1fb1843dd28a https://doi.org/10.1039/d2an00908k Zobrazit plný text záznamu