Determining the Effect of Pterostilbene on Insulin Secretion Using Chemoproteomics
| Autor: | Alessandra Tosco, Raffaele Riccio, Chiara Cassiano, Agostino Casapullo, Daniela Eletto, Maria Chiara Monti |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Proteomics
insulin Pterostilbene medicine.medical_treatment Pharmaceutical Science Resveratrol Chromatography Affinity Mass Spectrometry Article Exocytosis Analytical Chemistry SNARE complex lcsh:QD241-441 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine chemical proteomics lcsh:Organic chemistry Insulin Secretion Stilbenes Drug Discovery medicine Humans Chemoproteomics Physical and Theoretical Chemistry 030304 developmental biology 0303 health sciences Cheminformatics Insulin Organic Chemistry In vitro Bioavailability stilbene Biochemistry chemistry Chemistry (miscellaneous) Polyphenol 030220 oncology & carcinogenesis Molecular Medicine HeLa Cells Chemical proteomics Stilbene |
| Zdroj: | Molecules, Vol 25, Iss 2885, p 2885 (2020) Molecules Volume 25 Issue 12 |
| ISSN: | 1420-3049 |
| Popis: | Pterostilbene, the 3,5-dimethoxy derivative of resveratrol, is a well-known polyphenolic compound, mainly found in blueberries, grapevines, and Pterocarpus marsupium heartwood, which has recently attracted a great deal of attention due to its wide bio-pharmacological profile. Moreover, pterostilbene is more lipophilic than resveratrol, with a consequently better bioavailability and a more interesting therapeutic potential. In this work, a chemoproteomic approach, based on affinity chromatography, was applied on pterostilbene in the attempt to identify the biological targets responsible for its bioactivity. On this basis, syntaxins, a group of proteins involved in the formation of SNARE complexes mediating vesicles exocytosis, were selected among the most interesting pterostilbene interactors. In vitro and in cell assays gave evidence of the pterostilbene ability to reduce insulin secretion on glucose-stimulated pancreatic beta cells, opening the way to potential applications of pterostilbene as a supplement in the care of insulin-dependent metabolic disorders. |
| Databáze: | OpenAIRE |
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