DNA analysis of nosocomial infection by Enterobacter aerogenes in three cases of septicaemia in Japan
| Autor: | Takashi Yamamoto, Y. Aoki, H. Asakura, Hiroki Tsukada, Yukiko Tamura, Kyoko Ozaki, Fumitake Gejyo, Tatsuki Ohara, M. Itoh, Saori Nakagawa, Ikue Taneike, Seiichi Kojio, Satoshi Goshi |
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| Rok vydání: | 2002 |
| Předmět: |
Adult
DNA Bacterial Microbiology (medical) Microbial Sensitivity Tests Enterobacter aerogenes Ribotyping Microbiology Japan Sepsis Genotype Humans Typing Antibacterial agent Gel electrophoresis Cross Infection biology Enterobacteriaceae Infections General Medicine Middle Aged biology.organism_classification Virology Enterobacteriaceae Electrophoresis Gel Pulsed-Field Infectious Diseases Equipment Contamination Female Restriction digest Bacteria |
| Zdroj: | Journal of Hospital Infection. 51:221-225 |
| ISSN: | 0195-6701 |
| DOI: | 10.1053/jhin.2002.1255 |
| Popis: | Ceftazidime-resistant Enterobacter aerogenes was isolated from blood cultures of three patients with fever. DNA analysis using pulsed-field gel electrophoresis and ribosomal RNA gene restriction digest pattern analysis revealed that the strains were clonally similar to each other with a 79.3-96.0% homology. The same strain of E. aerogenes was isolated from a three-way stopcock connected to the indwelling catheter in one of the patients at a concentration of 45 cfu/mL. A similar strain was also isolated from the urine of one other patient on the same floor. The data suggest that E. aerogenes caused septicaemia via low bacterial contamination of a three-way stopcock in a peripheral drip intravenous infusion system in at least one patient, and that the outbreak of E. aerogenes infections was due to clonally-related strains. |
| Databáze: | OpenAIRE |
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