Engineering Bacillus thuringiensis bioinsecticides with an indigenous site-specific recombination system
| Autor: | C Gawron-Burke, J A Baum, M Kakefuda |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
endocrine system
Insecta Bacterial Toxins Bacillus thuringiensis Biology Applied Microbiology and Biotechnology Microbiology law.invention Hemolysin Proteins Plasmid Bacterial Proteins law Animals Replicon Site-specific recombination Pest Control Biological Gene Bacillus thuringiensis Toxins Ecology fungi Genetic transfer Parasporal body biology.organism_classification Recombinant Proteins Endotoxins Recombinant DNA Research Article Plasmids Food Science Biotechnology |
| Zdroj: | Applied and Environmental Microbiology. 62:4367-4373 |
| ISSN: | 1098-5336 0099-2240 |
| DOI: | 10.1128/aem.62.12.4367-4373.1996 |
| Popis: | The cry genes of Bacillus thuringiensis encode a diverse group of crystal-forming proteins that exhibit insecticidal activity, particularly against the larvae of lepidopteran, coleopteran, and dipteran insects. The efficacy of B. thuringiensis-based biopesticides may be improved through the genetic manipulation of these genes. A gene transfer system has been developed for the introduction and maintenance of cloned insecticidal cry genes on small plasmids in B. thuringiensis. This vector system combines a B. thuringiensis plasmid replicon and an indigenous site-specific recombination system that allows for the selective removal of ancillary or foreign DNA from the recombinant bacterium after introduction of the Cry-encoding plasmid. The site-specific recombination system is useful for engineering strains with unique combinations of cry genes, resulting in new active ingredients with improved insecticidal properties. |
| Databáze: | OpenAIRE |
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