Assessment of Multilocus Sequence Analysis (MLSA) for Identification of Candidatus Liberibacter Solanacearum from Different Host Plants in Spain
| Autor: | Verónica Pérez-Padilla, Leandro De León, Carmen Valor Martínez, Felipe Siverio, Raquel Marquínez, Eva García-Méndez, Jaime Cubero, Ana Ruiz-Padilla, Adrián Asensio, Cristina Redondo, Carmen Asensio-S.-Manzanera, Jerson Garita-Cambronero, Jesús Collar, José Luis Palomo, Ana Alfaro-Fernández |
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| Přispěvatelé: | European Commission, CSIC - Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Ruiz-Padilla, Ana, Garita-Cambronero, J., García-Méndez, E., Alfaro-Fernández, Ana, Asensio-S-Manzanera, C., Palomo, José Luis, Siverio, Felipe, de-León, Leandro, Cubero, Jaime |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Microbiology (medical) MLSA Citrus Candidatus Liberibacter zebra chip Sequence analysis Parsnips Single-nucleotide polymorphism 01 natural sciences Microbiology Zebra chip Article citrus 03 medical and health sciences Carrots Liberibacter Carrot Virology H20 Plant diseases Potatoes Gene Parsnip lcsh:QH301-705.5 carrot Genetics biology celery Haplotype Celery Ribosomal RNA biology.organism_classification Housekeeping gene HLB 030104 developmental biology lcsh:Biology (General) parsnip potato Potato 010606 plant biology & botany |
| Zdroj: | Microorganisms, Vol 8, Iss 1446, p 1446 (2020) electronico Microorganisms Volume 8 Issue 9 ReDivia. Repositorio Digital del Instituto Valenciano de Investigaciones Agrarias instname RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia ReDivia: Repositorio Digital del Instituto Valenciano de Investigaciones Agrarias Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| ISSN: | 2076-2607 |
| Popis: | 19 Pág. Liberibacter is a bacterial group causing different diseases and disorders in plants. Among liberibacters, Candidatus Liberibacter solanaceraum (CLso) produces disorders in several species mainly within Apiaceae and Solanaceae families. CLso isolates are usually grouped in defined haplotypes according to single nucleotide polymorphisms in genes associated with ribosomal elements. In order to characterize more precisely isolates of CLso identified in potato in Spain, a Multilocus Sequence Analysis (MLSA) was applied. This methodology was validated by a complete analysis of ten housekeeping genes that showed an absence of positive selection and a nearly neutral mechanism for their evolution. Most of the analysis performed with single housekeeping genes, as well as MLSA, grouped together isolates of CLso detected in potato crops in Spain within the haplotype E, undistinguishable from those infecting carrots, parsnips or celery. Moreover, the information from these housekeeping genes was used to estimate the evolutionary divergence among the different CLso by using the concatenated sequences of the genes assayed. Data obtained on the divergence among CLso haplotypes support the hypothesis of evolutionary events connected with different hosts, in different geographic areas, and possibly associated with different vectors. Our results demonstrate the absence in Spain of CLso isolates molecularly classified as haplotypes A and B, traditionally considered causal agents of zebra chip in potato, as well as the uncertain possibility of the present haplotype to produce major disease outbreaks in potato that may depend on many factors that should be further evaluated in future works. This research was funded by Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), grant numbers AT2016-007 and RTA2014-00008-C04-03-E, co-financed by FEDER. |
| Databáze: | OpenAIRE |
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