Effect of genotype, gelling agent, and auxin on the induction of somatic embryogenesis in sweet potato (Ipomoea batatas Lam.)
| Autor: | Vongthip Souvannavong, Robert Haïcour, Averil Chlyah, Darasinh Sihachakr, Zine El Abidine Triqui, Abdelkarim Guedira, Hassane Chlyah |
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| Rok vydání: | 2007 |
| Předmět: |
Somatic embryogenesis
Genotype Picloram Embryonic Development Biology Ipomoea General Biochemistry Genetics and Molecular Biology chemistry.chemical_compound Tissue culture Auxin Botany Ipomoea batatas chemistry.chemical_classification Ploidies General Immunology and Microbiology Indoleacetic Acids fungi Polysaccharides Bacterial food and beverages General Medicine biology.organism_classification Culture Media Agar chemistry Callus Kinetin Subculture (biology) General Agricultural and Biological Sciences |
| Zdroj: | Comptes rendus biologies. 331(3) |
| ISSN: | 1631-0691 |
| Popis: | Lateral buds of six cultivars of sweet potato were induced to form embryogenic callus in a culture medium solidified with two types of gelling agents, Agar or Gelrite, and supplemented with various concentrations of auxins, 2,4-D, 2,4,5-T and Picloram. Of the six cultivars screened, only three gave an embryogenic response. Best results with an average of 3.53% embryogenic response were obtained with the medium solidified with Agar, while in Gelrite only 0.45% of lateral buds gave rise to embryogenic callus. The interaction between the genotype and auxins was highly significant; particularly the optimal response was obtained with cv. Zho and 865 yielding 10.7 and 14.7% somatic embryogenesis, respectively, in the medium containing 2,4,5-T or Picloram. The plant conversion was dramatically improved by subculture of the embryogenic callus on the medium with the combination of 1 microM 2,4-D and 1 microM Kinetin or 5 microM ABA alone before transfer of mature embryos onto hormone-free medium. The embryogenic callus of sweet potato and its sustained ability to further regenerate plants have regularly been maintained for several years by frequent subculture in 5 microM 2,4,5-T or the combination of 10 microM 2,4-D and 1 microM BAP or kinetin. The embryo-derived plants seemed apparently genetically stable and similar to the hexaploid parental plants, based on morphological analysis and their ploidy level determined by using flow cytometry. |
| Databáze: | OpenAIRE |
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