Isolation and identification of an arabinogalactan extracted from pistachio external hull: Assessment of immunostimulatory activity
| Autor: | Manuel Vilanova, Elisabete Coelho, Sónia S. Ferreira, Mariem Hamed, Rita Bastos, Assaad Sila, Tânia Lima, Dmitry V. Evtuguin, Ali Bougatef, Manuel A. Coimbra |
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| Rok vydání: | 2021 |
| Předmět: |
chemistry.chemical_classification
Chromatography Size-exclusion chromatography Glycosidic bond General Medicine Nuclear magnetic resonance spectroscopy Structural characterization Galactans Analytical Chemistry Molecular Weight NMR spectroscopy chemistry Methylation analysis Immunostimulatory activity Arabinogalactan Polysaccharides Pistacia Sugar Two-dimensional nuclear magnetic resonance spectroscopy Arabinogalactan-protein Heteronuclear single quantum coherence spectroscopy Food Science Arabinogalactan protein |
| Zdroj: | Food chemistry. 373 |
| ISSN: | 1873-7072 |
| Popis: | This work studies the extraction and purification of a novel arabinogalactan from pistachio external hull. It was extracted with a simple method from pistachio hull which is considered as unexploited waste. Based on the results of sugar analysis by GC-FID, glycosidic linkage by GC–MS, NMR spectroscopy, and molecular weight by Size Exclusion Chromatography, pistachio hull water soluble polysaccharides (PHWSP) were identified as a type II arabinogalactan (AG), with characteristic terminally linked α-Araf, (α1 → 5)-Araf, (α1 → 3,5)-Araf, terminally linked β-Galp, (β1 → 6)-Galp, and (β1 → 3,6)-Galp. DEPT-135, HSQC, HMBC and COSY NMR data suggested the presence of (β1 → 3)-Galp mainly branched at O-6 with (β1 → 6)-Galp chains, α-Araf chains, and terminally linked α-Araf. These AG from pistachio external hulls showed in vitro stimulatory activity for B cells, suggesting their possible use as an immunological stimulant in nutraceutical and biomedical applications. |
| Databáze: | OpenAIRE |
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