Effects of UVA+UVB solar radiation levels and cross-linking on animal model with Bowman’s layer: Chicken
| Autor: | Gisele Cristina Gonçalves, Lucía Ibares Frías, Santiago Mar Sardaña, María del Carmen Martínez García |
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| Rok vydání: | 2019 |
| Předmět: |
Ultraviolet radiation
integumentary system Radiación ultravioleta Chemistry Bowman’s layer 2209.15 Optometría Técnica de cross-linking Radiation eye diseases Córnea medicine.anatomical_structure Animal model Cornea medicine Biophysics sense organs Cross-linking technique Membrana de Bowman Bowman's layer |
| Zdroj: | UVaDOC. Repositorio Documental de la Universidad de Valladolid instname UVaDOC: Repositorio Documental de la Universidad de Valladolid Universidad de Valladolid |
| Popis: | Producción Científica Purpose: Evaluate the corneal damage and wound healing in an animal model with Bowman’s layer after UVA+UVB radiation and epioff Cross-Linking (CXL). Methods: Adult Brown Classic chickens were randomly divided into three groups. The UVA+UVB group (20 eyes) was exposed to three doses of radiation (4.60 J/cm2) . The CXL group (12 eyes), was de-epithelialized and irradiated with UVA light (5.4 J/cm2) for 30 minutes. The contralateral eyes were used as a control (12 eyes). The animals have clinically follow-up, the epithelial closure was measured by fluorescein stain and corneal thickness by pachymetry. Keratocytes loss, subsequent cell repopulation, and healing events were analyzed after euthanasia at 24 hours, 15 and 30 days post-radiation on hematoxylin-eosin-stained sections. Cell death was detected by TUNEL assay, proliferation by BrdU (bromodeoxyuridine) immunofluorescence, and myofibroblasts differentiation by (αSMA) immunohistochemistry. Results: Re-epithelialization was at 5 ± 2.3 days in CXL and at day 2 ± 0.9 days in UVA+UVB. Pachymetry was higher after UVA+UVB than CXL (p |
| Databáze: | OpenAIRE |
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