Advanced oxidation protein products aggravate cardiac remodeling via cardiomyocyte apoptosis in chronic kidney disease
Autor: | Kun Zhang, Qingqing Cai, Jingfeng Wang, Yinyin Zhang, Yu Liu, Weijing Feng, Hui Huang, Mong Heng Wang, Wanbing He He, Jie Chen |
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Rok vydání: | 2017 |
Předmět: |
0301 basic medicine
Male medicine.medical_specialty Heart Diseases Physiology Apoptosis Biology Antioxidants Cell Line Rats Sprague-Dawley 03 medical and health sciences Physiology (medical) Internal medicine medicine Animals Myocytes Cardiac Renal Insufficiency Chronic Ventricular Remodeling Endoplasmic reticulum JNK Mitogen-Activated Protein Kinases Acetophenones medicine.disease Endoplasmic Reticulum Stress Cell biology Up-Regulation Disease Models Animal Oxidative Stress 030104 developmental biology Endocrinology Advanced oxidation protein products Advanced Oxidation Protein Products Cardiology and Cardiovascular Medicine Cardiomyocyte apoptosis Kidney disease Signal Transduction |
Zdroj: | American journal of physiology. Heart and circulatory physiology. 314(3) |
ISSN: | 1522-1539 |
Popis: | Advanced oxidation protein products (AOPPs) are independent risk factor for various cardiovascular diseases. Cardiomyocyte apoptosis has been implicated as an important mechanism in cardiac remodeling in chronic kidney disease (CKD). However, whether AOPPs affect cardiomyocyte apoptosis and subsequent cardiac remodeling in CKD is still not very clear. Here, we assessed the role of AOPPs in cardiomyocyte apoptosis in CKD. H9C2 rat cardiomyoblast cells were exposed to AOPPs. Apoptotic cells were determined by TUNEL assay. The expression of apoptotic markers (cleaved caspase-3 and Bax), JNK signaling, and endoplasmic reticulum stress were explored. Serum AOPPs were measured in male Sprague-Dawley rats that underwent sham surgery and 5/6 nephrectomy, respectively. In vitro, our findings showed that AOPPs activated JNK signaling and endoplasmic reticulum stress and significantly aggravated H9C2 rat cardiomyoblast cells apoptosis. These effects were partially ameliorated by apocynin with inhibition of oxidative stress. In vivo, serum levels of AOPPs were progressively elevated with the increasing time course in CKD rats compared with sham-operated rats ( P0.05). Serum AOPP levels were positively associated with cardiomyocyte apoptosis ( R |
Databáze: | OpenAIRE |
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