Identification, cloning and characterization of a novel nuclear protein, HA95, homologous to A-kinase anchoring protein 95*
| Autor: | Sigurd Ørstavik, Philippe Collas, Tore Jahnsen, Elliott Kieff, Turid Eide, Inn-oc Han, Bjørn Steen Sklhegg, Kjetil Taskén |
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| Rok vydání: | 2000 |
| Předmět: |
A-kinase-anchoring protein
DNA Complementary Molecular Sequence Data Biology Antibodies Cell Line Proto-Oncogene Proteins c-myc HSPA2 Cyclic AMP Animals Humans Amino Acid Sequence RNA Messenger Cloning Molecular Nuclear protein Muscle Skeletal Nuclear export signal Peptide sequence B-Lymphocytes Nuclear cap-binding protein complex Intracellular Signaling Peptides and Proteins Nuclear Proteins Exons Cell Biology General Medicine Blotting Northern Cyclic AMP-Dependent Protein Kinases Molecular biology Cell biology DNA-Binding Proteins Nuclear lamina Rabbits Nuclear localization sequence Protein Binding |
| Zdroj: | Biology of the Cell. 92:27-37 |
| ISSN: | 0248-4900 |
| DOI: | 10.1016/s0248-4900(00)88761-4 |
| Popis: | Previously, we have identified and characterized nuclear AKAP95 from man which targets cyclic AMP (cAMP)-dependent protein kinase (PKA)-type II to the condensed chromatin/spindle region at mitosis. Here we report the cloning of a novel nuclear protein with an apparent molecular mass of 95 kDa that is similar to AKAP95 and is designated HA95 (homologous to AKAP95). HA95 cDNA sequence encodes a protein of 646 amino acids that shows 61% homology to the deduced amino acid sequence of AKAP95. The HA95 gene is located on chromosome 19p13.1 immediately upstream of the AKAP95 gene. Both HA95 and AKAP95 genes contain 14 exons encoding similar regions of the respective proteins, indicating a previous gene duplication event as the origin of the two tandem genes. Despite their apparent similarity, HA95 does not bind RII in vitro. HA95 contains a putative nuclear localization signal in its N-terminal domain. It is localized exclusively into the nucleus as demonstrated in cells transfected with HA95 fused to either green fluorescence protein or the c-myc epitope. In the nucleus, the HA95 protein is found as complexes directly associated with each other or indirectly associated via other nuclear proteins. In interphase, HA95 is co-localized with AKAP95, but the two proteins are not biochemically associated. At metaphase, both proteins co-localize with condensed chromosomes. The similarity in sequence and localization of HA95 and AKAP95 suggests that the two molecules constitute a novel family of nuclear proteins that may exhibit related functions. |
| Databáze: | OpenAIRE |
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