The Hob proteins are novel and conserved lipid-binding proteins at ER-PM contact sites
| Autor: | Arash Bashirullah, Scott D. Emr, Jeremy T. Smyth, Sarah D. Neuman, Jeff R Jorgensen, Amy T. Cavanagh, Jane E. Selegue |
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| Rok vydání: | 2021 |
| Předmět: |
Endoplasmic reticulum
Cell Membrane Vesicular Transport Proteins Membrane Proteins Cell Biology Protein composition Saccharomyces cerevisiae Mutant cell Biology Endoplasmic Reticulum Phosphatidylinositols Yeast Cell biology chemistry.chemical_compound Membrane Drosophila melanogaster chemistry Lipid binding Organelle Animals Drosophila Proteins Phosphatidylinositol Carrier Proteins Research Article |
| Zdroj: | J Cell Sci |
| ISSN: | 1477-9137 |
| Popis: | Membrane contact sites are critical junctures for organelle signaling and communication. Endoplasmic reticulum–plasma membrane (ER–PM) contact sites were the first membrane contact sites to be described; however, the protein composition and molecular function of these sites is still emerging. Here, we leverage yeast and Drosophila model systems to uncover a novel role for the Hobbit (Hob) proteins at ER–PM contact sites. We find that Hobbit localizes to ER–PM contact sites in both yeast cells and the Drosophila larval salivary glands, and this localization is mediated by an N-terminal ER membrane anchor and conserved C-terminal sequences. The C-terminus of Hobbit binds to plasma membrane phosphatidylinositols, and the distribution of these lipids is altered in hobbit mutant cells. Notably, the Hobbit protein is essential for viability in Drosophila, providing one of the first examples of a membrane contact site-localized lipid binding protein that is required for development. |
| Databáze: | OpenAIRE |
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