Dynamic acoustic field activated cell separation (DAFACS)
| Autor: | J. N. Roberts, David R. S. Cumming, G. D. Skotis, Anne L. Bernassau, Mathis O. Riehle |
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| Rok vydání: | 2015 |
| Předmět: |
Neurons
Range (particle radiation) education.field_of_study Materials science Particle number Swine Microfluidics Population Biomedical Engineering Bioengineering Classical fluids Nanotechnology Acoustics Cell Separation General Chemistry Biochemistry Ganglia Spinal Tweezers Animals Particle Particle size Particle Size education Biological system |
| Zdroj: | Lab on a Chip. 15:802-810 |
| ISSN: | 1473-0189 1473-0197 |
| DOI: | 10.1039/c4lc01153h |
| Popis: | Advances in diagnostics, cell and stem cell technologies drive the development of application-specific tools for cell and particle separation. Acoustic micro-particle separation offers a promising avenue for high-throughput, label-free, high recovery, cell and particle separation and isolation in regenerative medicine. Here, we demonstrate a novel approach utilizing a dynamic acoustic field that is capable of separating an arbitrary size range of cells. We first demonstrate the method for the separation of particles with different diameters between 6 and 45 μm and secondly particles of different densities in a heterogeneous medium. The dynamic acoustic field is then used to separate dorsal root ganglion cells. The shearless, label-free and low damage characteristics make this method of manipulation particularly suited for biological applications. Advantages of using a dynamic acoustic field for the separation of cells include its inherent safety and biocompatibility, the possibility to operate over large distances (centimetres), high purity (ratio of particle population, up to 100%), and high efficiency (ratio of separated particles over total number of particles to separate, up to 100%). |
| Databáze: | OpenAIRE |
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