The relationship between COL3A1 exon 31 polymorphism and pelvic organ prolapse
| Autor: | Jong Rak Choi, Sue Min Chung, Sei Kwang Kim, Hyun Joo Jung, Myung Jae Jeon, Sang Wook Bai |
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| Rok vydání: | 2008 |
| Předmět: |
Nephrology
Stress incontinence medicine.medical_specialty Urology Single-nucleotide polymorphism Gastroenterology Exon Uterine Prolapse Internal medicine Genotype Medicine Humans Gynecology Polymorphism Genetic business.industry Exons Rectal Prolapse Pelvic cavity Middle Aged medicine.disease Menopause medicine.anatomical_structure Collagen Type III Female Restriction fragment length polymorphism business Cystocele |
| Zdroj: | The Journal of urology. 181(3) |
| ISSN: | 1527-3792 |
| Popis: | We investigated the role of COL3A1 exon 31 polymorphism (a single base substitution from guanine to adenine at +2092), resulting in the replacement of alanine with threonine at the 698th amino acid of COL3A1, in the pathogenesis of pelvic organ prolapse.A total of 72 postmenopausal Korean women who were not on hormonal replacement therapy and who had a history of vaginal childbirth were enrolled in this study. The patient group consisted of 36 women diagnosed with stage II or greater pelvic organ prolapse irrespective of urodynamic stress incontinence. The control group consisted of 36 healthy volunteers with pelvic organ prolapse quantification system stage 0 or I disease without urodynamic stress incontinence. After extracting the genomic DNA from peripheral blood leukocytes the polymorphism of exon 31 of COL3A1 was typed by restriction fragment length polymorphism (Alu I restriction fragment length polymorphism) and confirmed by direct sequencing.Frequency of the G allele was significantly higher in patients with pelvic organ prolapse than in controls (0.8 vs 0.6, p = 0.002). In women with the G allele the OR for pelvic organ prolapse was 3.2 (95% CI 1.4-7.3).COL3A1 exon 31 polymorphism may have a role in determining the risk of pelvic organ prolapse in women with risk factors such as aging, vaginal childbirth and hypoestrogenism. |
| Databáze: | OpenAIRE |
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