Expression of horse and donkey LH in COS-7 cells : evidence for low FSH activity in donkey LH compared with horse LH
| Autor: | Nadine Martinat, Yves Combarnous, H Marichatou, M Chopineau, Carine Troispoux, Florian Guillou, F Stewart |
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| Přispěvatelé: | Unité de recherche Physiologie de la reproduction des mammifères domestiques, Nouzilly, Institut National de la Recherche Agronomique (INRA), ProdInra, Migration |
| Jazyk: | angličtina |
| Rok vydání: | 1997 |
| Předmět: |
LH
endocrine system medicine.medical_specialty Gonadotropins Equine medicine.drug_class Endocrinology Diabetes and Metabolism [SDV]Life Sciences [q-bio] Biological Availability Heterologous 030209 endocrinology & metabolism [INFO] Computer Science [cs] Biology 03 medical and health sciences Follicle-stimulating hormone 0302 clinical medicine Endocrinology Internal medicine FSH medicine Animals Bioassay SOUS-UNITE [INFO]Computer Science [cs] Horses STRUCTURE-ACTIVITE ComputingMilieux_MISCELLANEOUS 030304 developmental biology 0303 health sciences Leydig cell BIOCHIMIE Horse Equidae Luteinizing Hormone ANE Recombinant Proteins [SDV] Life Sciences [q-bio] medicine.anatomical_structure COS Cells Biological Assay Follicle Stimulating Hormone Gonadotropin Luteinizing hormone Follicle-stimulating hormone receptor hormones hormone substitutes and hormone antagonists |
| Zdroj: | Journal of Endocrinology Journal of Endocrinology, BioScientifica, 1997, 152, pp.371-377 |
| ISSN: | 0022-0795 1479-6805 |
| Popis: | Horse (Equus caballus) luteinizing hormone (eLH) and chorionic gonadotrophin (eCG), which have the same amino acid sequence, are unusual in that, although they express only LH activity in equids, they express dual LH and FSH activities in all other species tested. Donkey (Equus asinus) LH (dkLH) and CG (dkCG), which also share an identical peptide backbone, have been less well characterized and conflicting results concerning their FSH activity in heterologous species have appeared in the literature. In order to assess and compare the intrinsic LH and FSH activities of the horse and donkey LHs in heterologous species, recombinant eLH (r.eLH/CG) and recombinant dkLH (r.dkLH/CG) were expressed, for the first time, in COS-7 cells. Their LH activities were assessed in a rat Leydig cell bioassay, and their FSH activities were estimated in a bioassay using Y1 cells stably expressing the human FSH receptor. Human CG (hCG) was expressed (r.hCG) and analysed in the same system. The results showed that, whereas r.dkLH/CG was about twice as active as r.eLH/CG in the LH bioassay, it was five times less active than r.eLH/CG in the FSH bioassay; r.hCG was about three times less active than r.eLH/CG in the LH bioassay but was completely inactive in the FSH bioassay. These results confirm that dkLH/CG possesses significant FSH activity in heterologous species that is not attributable to contamination with FSH. Journal of Endocrinology (1997) 152, 371–377 |
| Databáze: | OpenAIRE |
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