Recombinant human antibodies specific for the Pfs48/45 protein of the malaria parasite Plasmodium falciparum
| Autor: | Wijnand Eling, Will Roeffen, Jos M.H. Raats, Karina Teelen, Walther J. van Venrooij, Rene Hoet, Robert W. Sauerwein |
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| Rok vydání: | 2001 |
| Předmět: |
Phage display
Sequence analysis Molecular Sequence Data Plasmodium falciparum Protozoan Proteins gastheer-parasiet interactie [Malaria] chemical and pharmacologic phenomena parasite-host interaction [Malaria] Biochemistry Epitope Antibodies law.invention Epitopes law Gametocyte Parasite hosting Animals Humans Bacteriophages Amino Acid Sequence Molecular Biology Membrane Glycoproteins biology Sequence Homology Amino Acid Cell Biology respiratory system biology.organism_classification Virology Molecular biology Recombinant Proteins Recombinant DNA biology.protein Antibody |
| Zdroj: | Journal of Biological Chemistry, 276, 23, pp. 19807-11 Journal of Biological Chemistry, 276, 19807-11 |
| ISSN: | 1083-351X |
| Popis: | Contains fulltext : 216407.pdf (Publisher’s version ) (Open Access) We report the first construction of two combinatorial human phage display libraries derived from malaria-immune patients. Specific single-chain Fv fragments (scFv) against Pfs48/45, a gamete surface protein of the sexual stages of Plasmodium falciparum, were selected and analyzed extensively. The selected scFv reacted with the surface of extracellular sexual forms of the parasite and showed Pfs48/45 reactivity on immunoblot. The scFv inhibit binding of human malaria sera to native Pfs48/45 from gametocytes. Moreover, the scFv bind to target epitopes of Pfs48/45 exposed in natural infections. Sequence analysis of eight scFv clones specific for epitope III of Pfs48/45 revealed that these clones could be divided into one V(H) family-derived germ-line gene (V(H)1) and two V(L) family segments (V(L)2 and V(K)I). |
| Databáze: | OpenAIRE |
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