Ethylene glycol dimethacrylate and diethylene glycol dimethacrylate exhibits cytotoxic and genotoxic effect on human gingival fibroblasts via induction of reactive oxygen species
| Autor: | Ireneusz Majsterek, Piotr Czarny, Renata Krupa, Ewelina Synowiec, Bartosz Kowalski, Janusz Szemraj, Paulina Wigner, Malgorzata Drzewiecka, Monika Toma, Marzena Szwed, Tomasz Sliwinski, Anna Bielecka-Kowalska, Michał Kowalski |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
DNA Repair DNA damage Cell Survival Ethylene glycol dimethacrylate Gingiva Apoptosis Dental technician Toxicology 03 medical and health sciences chemistry.chemical_compound Dental Materials 0302 clinical medicine Humans MTT assay Cells Cultured chemistry.chemical_classification Reactive oxygen species Glutathione Peroxidase Superoxide Dismutase Osmolar Concentration Diethylene glycol G1 Phase people.profession 030206 dentistry General Medicine Phospholipid Hydroperoxide Glutathione Peroxidase Comet assay Oxidative Stress 030104 developmental biology Cross-Linking Reagents Biochemistry chemistry Methacrylic acid Enzyme Induction Methacrylates Ethylene Glycols Comet Assay people Reactive Oxygen Species DNA Damage Plasmids |
| Zdroj: | Toxicology in vitro : an international journal published in association with BIBRA. 47 |
| ISSN: | 1879-3177 |
| Popis: | Although methacrylic acid derivatives in their polymeric form are considered to be safe, insufficient polymerization and the release of monomers due to either mechanical or enzymatical factors can lead to their reaching millimolar concentrations in local tissue. The present study evaluates the effect of two methacrylate monomers - ethylene glycol dimethacrylate (EGDMA) and diethylene glycol dimethacrylate (DEGDMA) - on human gingival fibroblasts (HGFs). Both monomers were found to reduce cells viability in MTT assay, increase apoptosis and cause cell cycle arrest in G1/G0 phase. They also increased intracellular reactive oxygen species (ROS) production as measured by DCFH-DA and DHE probes and increased expression of GPx4 and SOD2. Both monomers increased DNA damage in comet assay. Moreover, HGFs were not able to repair those lesions within 120min of repair incubation. However, the monomers were not found to have any effect on the integrity of isolated plasmids. We postulate that EGDMA and DEGDMA exhibit their cytotoxic and genotoxic properties via increased production of ROS, which cause DNA damage, affect apoptosis, viability and cell cycle. Further studies are needed to better understand the properties of methacrylic acid monomers and to evaluate the risk that they cause for patients, dentists and dental technicians. |
| Databáze: | OpenAIRE |
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