Evaluation of eight RNA isolation methods for transcriptional analysis in Campylobacter jejuni

Autor:	Viraj N. Perera, Vongsavanh Phongsisay, Benjamin N. Fry
Rok vydání:	2007
Předmět:	Microbiology (medical) Regulation of gene expression Deoxyribonucleases biology Reverse Transcriptase Polymerase Chain Reaction RNA biology.organism_classification Microbiology Campylobacter jejuni Molecular biology law.invention Reverse transcription polymerase chain reaction RNA Bacterial law Bacteriology Spectrophotometry Ultraviolet RNA extraction Molecular Biology Polymerase chain reaction Bacteria
Zdroj:	Journal of Microbiological Methods. 68:427-429
ISSN:	0167-7012
DOI:	10.1016/j.mimet.2006.09.002
Popis:	DNA-free RNA samples are essential to investigate gene regulation using real-time RT-PCR. This study evaluated eight RNA isolation methods in combination with TURBO DNase treatment and acid phenol extractions for their ability to produce DNA-free RNA from Campylobacter jejuni strains.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::df9fa3032185f75362874b3cc0e8882d https://doi.org/10.1016/j.mimet.2006.09.002 Zobrazit plný text záznamu Full Text from ScienceDirect