In vitro antioxidant and cytotoxic activity of extracts of Baccharis coridifolia DC
| Autor: | J. Coussio, G. Ciccia, J. Rodriguez Talou, E. Mongelli, C. Desmarchelier |
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| Rok vydání: | 1997 |
| Předmět: |
Male
Antioxidant medicine.medical_treatment In Vitro Techniques Pharmacognosy Biology Thiobarbituric Acid Reactive Substances Antioxidants KB Cells chemistry.chemical_compound Drug Discovery medicine TBARS Animals Humans Bioassay Rats Wistar IC50 Dichloromethane Pharmacology Plants Medicinal Chromatography Plant Extracts Baccharis Biological activity Hydrogen Peroxide South America Oxidants biology.organism_classification Antineoplastic Agents Phytogenic Rats Liver Biochemistry chemistry Luminescent Measurements Drug Screening Assays Antitumor DNA Damage |
| Zdroj: | Journal of Ethnopharmacology. 58:157-163 |
| ISSN: | 0378-8741 |
| DOI: | 10.1016/s0378-8741(97)00106-2 |
| Popis: | The in vitro antioxidant and cytotoxic activities in different extracts of Baccharis coridifolia DC. (Compositae) were studied using different bioassays. Antioxidant activity was tested using hydroperoxide-initiated chemiluminescence (CL) in rat liver homogenates. The aqueous extract showed antioxidant activity (IC50 = 141 microg/ml), while the dichloromethane and methanol extracts were prooxidant. Activity in the aqueous extract was confirmed by determination of thiobarbituric acid-reactive substances (TBARS), showing an IC50 = 556 microg/ml. On the other hand, studies on the cytotoxic activity demonstrated a 78% crown gall tumour inhibition and an ED50 = 4. 2 microg/ml in KB cells growth for the dichloromethane extract. A 56% decrease in the initial absorbance of DNA-Methyl Green complex could also be observed at 1000 microg/ml, suggesting the presence of compounds that interact with DNA. |
| Databáze: | OpenAIRE |
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