Extensive in vitro gastrointestinal digestion markedly reduces the immune-toxicity of Triticum monococcum wheat: implication for celiac disease
| Autor: | Gianfrani, Carmen, Camarca, Alessandra, Mazzarella, Giuseppe, Di stasio, Luigia, Giardullo, Nicola, Ferranti, Pasquale, Picariello, Gianluca, Rotondi aufiero, Vera, Picascia, Stefania, Troncone, Riccardo, Pogna, Norberto, Auricchio, Salvatore, Mamone, Gianfranco |
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| Přispěvatelé: | Gianfrani, C, Camarca, A, Mazzarella, G, Stasio, Ld, Giardullo, N, Ferranti, Pasquale, Picariello, G, Aufiero, Vr, Picascia, S, Troncone, Riccardo, Pogna, N, Auricchio, S, Mamone, G. |
| Rok vydání: | 2015 |
| Předmět: |
Proteolysis
T-Lymphocytes Brush border membrane Celiac disease Gastrointestinal digestion Gliadin Triticum monococcum Epitope Microbiology Cell Line Immune system Tandem Mass Spectrometry Botany medicine Humans Triticum chemistry.chemical_classification medicine.diagnostic_test biology nutritional and metabolic diseases food and beverages digestive system diseases In vitro Gastrointestinal Tract Celiac Disease Enzyme chemistry biology.protein Ploidy Digestion Peptides Food Science Biotechnology Chromatography Liquid |
| Zdroj: | Molecular nutrition & food research 59 (2015): 1844–1854. doi:10.1002/mnfr.201500126 info:cnr-pdr/source/autori:Gianfrani, Carmen; Gianfrani, Carmen; Camarca, Alessandra; Mazzarella, Giuseppe; Di stasio, Luigia; Giardullo, Nicola; Ferranti, Pasquale; Ferranti, Pasquale; Picariello, Gianluca; Rotondi aufiero, Vera; Picascia, Stefania; Troncone, Riccardo; Pogna, Norberto; Auricchio, Salvatore; Mamone, Gianfranco/titolo:Extensive in vitro gastrointestinal digestion markedly reduces the immune-toxicity of Triticum monococcum wheat: Implication for celiac disease/doi:10.1002%2Fmnfr.201500126/rivista:Molecular nutrition & food research (Print)/anno:2015/pagina_da:1844/pagina_a:1854/intervallo_pagine:1844–1854/volume:59 |
| ISSN: | 1613-4133 |
| Popis: | cope The ancient diploid Triticum monococcum is of special interest as a candidate low-toxic wheat species for celiac disease patients. Here, we investigated how an in vitro gastro-intestinal digestion, affected the immune toxic properties of gliadin from diploid compared to hexaploid wheat. Methods and results Gliadins from Triticum monococcum, and Triticum aestivum cultivars were digested using either a partial proteolysis with pepsin-chymotrypsin, or an extensive degradation that used gastrointestinal enzymes including the brush border membrane enzymes. The immune stimulatory properties of the digested samples were investigated on T-cell lines and jejunal biopsies from celiac disease patients. The T-cell response profile to the Triticum monococcum gliadin was comparable to that obtained with Triticum aestivum gliadin after the partial pepsin-chymotrypsin digestion. In contrast, the extensive gastrointestinal hydrolysis drastically reduced the immune stimulatory properties of Triticum monococcum gliadin. MS-based analysis showed that several Triticum monococcum peptides, including known T-cell epitopes, were degraded during the gastrointestinal treatment, whereas many of Triticum aestivum gliadin survived the gastrointestinal digestion. Conclusion The pattern of Triticum monococcum gliadin proteins is sufficiently different from those of common hexaploid wheat to determine a lower toxicity in celiac disease patients following in vitro simulation of human digestion. |
| Databáze: | OpenAIRE |
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