Antiviral Action of Synthetic Stigmasterol Derivatives on Herpes Simplex Virus Replication in Nervous Cells In Vitro
| Autor: | Erina Petrera, Laura Edith Alche, Analía Gabriela Níttolo |
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| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Article Subject
Otras Ciencias Biológicas Anti-Inflammatory Agents Stigmasterol lcsh:Medicine IN VITRO Herpesvirus 1 Human Biology medicine.disease_cause Virus Replication Antiviral Agents General Biochemistry Genetics and Molecular Biology Virus Microbiology Cell Line purl.org/becyt/ford/1 [https] Ciencias Biológicas chemistry.chemical_compound Interferon-gamma Mice NERVOUS CELLS Chlorocebus aethiops medicine Animals Humans Secretion Cytotoxicity purl.org/becyt/ford/1.6 [https] Vero Cells Inflammation General Immunology and Microbiology Interleukin-6 lcsh:R HSV Epithelial Cells General Medicine medicine.disease Virology In vitro Cholestanones Herpes simplex virus chemistry Cell culture SYNTHETIC STIGMASTEROL DERIVATIVES Encephalitis CIENCIAS NATURALES Y EXACTAS Research Article |
| Zdroj: | BioMed Research International CONICET Digital (CONICET) Consejo Nacional de Investigaciones Científicas y Técnicas instacron:CONICET BioMed Research International, Vol 2014 (2014) |
| ISSN: | 2314-6141 2314-6133 |
| Popis: | Polyfunctionalized stigmasterol derivatives, (22S,23S)-22,23-dihydroxystigmast-4-en-3-one (compound 1) and (22S,23S)-3β-bromo-5α,22,23-trihydroxystigmastan-6-one (compound 2), inhibit herpes simplex virus type 1 (HSV-1) replication and spreading in human epithelial cells derived from ocular tissues. Both compounds reduce the incidence and severity of lesions in a murine model of herpetic stromal keratitis when administered in different treatment modalities. Since encephalitis caused by HSV-1 is another immunopathology of viral origin, we evaluate here the antiviral effect of both compounds on HSV-1 infected nervous cell lines as well as their anti-inflammatory action. We found that both stigmasterol derivatives presented low cytotoxicity in the three nervous cell lines assayed. Regarding the antiviral activity, in all cases both compounds prevented HSV-1 multiplication when added after infection, as well as virus propagation. Additionally, both compounds were able to hinder interleukin-6 and Interferon-gamma secretion induced by HSV-1 infection in Neuro-2a cells. We conclude that compounds 1 and 2 have exerted a dual antiviral and anti-inflammatory effect in HSV-1 infected nervous cell lines, which makes them interesting molecules to be further studied. Fil: Petrera, Erina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina Fil: Níttolo, Analía Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina Fil: Alche, Laura Edith. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina |
| Databáze: | OpenAIRE |
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