The methylotrophic yeast Hansenula polymorpha: a versatile cell factory
| Autor: | R. B. Van Dijk, J.A.K.W. Kiel, Marten Veenhuis, I.J. van der Klei, Klaas Nico Faber |
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| Přispěvatelé: | Faculteit Medische Wetenschappen/UMCG, Groningen Institute for Gastro Intestinal Genetics and Immunology (3GI), Center for Liver, Digestive and Metabolic Diseases (CLDM) |
| Rok vydání: | 2000 |
| Předmět: |
Glycosylation
Autonomously replicating sequence HEPATITIS-B Saccharomyces cerevisiae genetic tools Heterologous heterologous proteins Bioengineering Biology AUTONOMOUS REPLICATION Applied Microbiology and Biotechnology Biochemistry Insert (molecular biology) Pichia pastoris Microbiology Hansenula polymorpha SACCHAROMYCES-CEREVISIAE chemistry.chemical_compound MATRIX PROTEIN ALPHA-GALACTOSIDASE biology.organism_classification TRANSFORMATION Yeast PEROXISOME BIOGENESIS ENVIRONMENTAL-CONDITIONS PICHIA-PASTORIS Membrane protein chemistry HETEROLOGOUS GENE-EXPRESSION cell factory Biotechnology |
| Zdroj: | Enzyme and Microbial Technology, 26(9-10), 793-800. ELSEVIER SCIENCE INC |
| ISSN: | 0141-0229 |
| DOI: | 10.1016/s0141-0229(00)00173-3 |
| Popis: | The development of heterologous overexpression systems for soluble proteins has greatly advanced the study of the structure/function relationships of these proteins and their biotechnological and pharmaceutical applications. In this paper we present an overview on several aspects of the use of the methylotrophic yeast Hansenula polymorpha as a host for heterologous gene expression. H. polymorpha has been successfully exploited as a cell factory for the large-scale production of such components. Stable, engineered strains can be obtained by site-directed integration of expression cassettes into the genome, for which various constitutive and inducible promoters are available to control the expression of the foreign genes. New developments have now opened the way to additional applications of H. polymorpha, which are unprecedented for other organisms. Most importantly, it may be the organism of choice for reliable, large-scale production of heterologous membrane proteins, using inducible intracellular membranes and targeting sequences to specifically insert these proteins stably into these membranes. Furthermore, the use of H. polymorpha offers the possibility to accumulate the produced components into specific compartments, namely peroxisomes. These organelles are massively induced during growth of the organism on methanol and may occupy up to 80% of the cell volume. Accumulation inside peroxisomes prevents undesired modifications (e.g. proteolytic processing or glycosylation) and is also in particular advantageous when proteins are produced which are toxic or harmful for the host. (C) 2000 Elsevier Science Inc. All rights reserved. |
| Databáze: | OpenAIRE |
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