Strontium substituted biomimetic calcium phosphate system derived from cuttlefish bone
| Autor: | Matija Cvetnić, Antonia Ressler, Hrvoje Ivanković, Inga Marijanović, Maja Antunović, Marica Ivanković |
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| Rok vydání: | 2020 |
| Předmět: |
Calcium Phosphates
Materials science Simulated body fluid Biomedical Engineering chemistry.chemical_element 02 engineering and technology Calcium 010402 general chemistry 01 natural sciences Bone and Bones Biomaterials chemistry.chemical_compound Biomimetic Materials Materials Testing Animals Humans co-substitution hydroxyapatite octacalcium phosphate Rietveld refinement strontium Amorphous calcium phosphate Octacalcium phosphate Strontium Decapodiformes 021001 nanoscience & nanotechnology Phosphate 0104 chemical sciences HEK293 Cells Calcium carbonate chemistry 0210 nano-technology Nuclear chemistry |
| Zdroj: | Journal of Biomedical Materials Research Part B: Applied Biomaterials. 108:1697-1709 |
| ISSN: | 1552-4981 1552-4973 |
| DOI: | 10.1002/jbm.b.34515 |
| Popis: | Biomimetic triphasic strontium‐substituted calcium phosphate (CaP) powders were prepared by wet precipitation method at 50°C, using CaCO3, (NH2)2COH3PO4, and Sr(NO3)2 as reagents. Calcite was prepared from biogenic source (cuttlefish bone). The synthesized powders have been characterized by elemental analysis, Fourier transform infrared spectrometry, X‐ray diffraction, Rietveld refinement studies and cell viability test. Phase transformation and ion release were analyzed during 7 days of incubation in simulated body fluid at 37°C. The raw precipitated powders were composed of calcium deficient carbonated hydroxyapatite (HA), octacalcium phosphate (OCP), and amorphous calcium phosphate (ACP). After heat treatment at 1200°C β‐tricalcium phosphate (β‐TCP) was detected. Strontium substitution for calcium results in an increase of lattice parameters in HA, OCP, and β‐TCP. Sr2+ occupy the Ca(1) site in HA, Ca(3, 4, 7, 8) sites in OCP and Ca(1, 2, 3, 4) sites in β‐TCP. Along with Sr2+ substitution, presence of Mg2+ and Na+ ions was detected as a result of using biogenic calcium carbonate. The culture of human embryonic kidney cells indicated noncytotoxicity of the prepared CaP powders with emphasis on the cell proliferation during 3 days of culture. |
| Databáze: | OpenAIRE |
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