Drilling Combined with Adipose-derived Stem Cells and Bone Morphogenetic Protein-2 to Treat Femoral Head Epiphyseal Necrosis in Juvenile Rabbits
| Autor: | Rongzhen He, Cheng Peng, Xu Cao, Bin Tu, Zili Wang, Jinshen He, Kun Xiong, Ba Hongliang, Hansong Xia, Song Wu |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Male Pathology medicine.medical_specialty H&E stain Adipose tissue Bone Morphogenetic Protein 2 Biochemistry Bone morphogenetic protein 2 03 medical and health sciences chemistry.chemical_compound Femoral head 0302 clinical medicine Femur Head Necrosis Genetics Medicine Animals Orthopedic Procedures Growth Plate Bone regeneration Cell Shape Cell Proliferation 030222 orthopedics business.industry Stem Cells Femur Head Magnetic Resonance Imaging 030104 developmental biology medicine.anatomical_structure chemistry Adipose Tissue Bromodeoxyuridine Epiphysis Rabbits Stem cell business Epiphyses Stem Cell Transplantation |
| Zdroj: | Current medical science. 38(2) |
| ISSN: | 2523-899X |
| Popis: | This study was designed to evaluate the effects of drilling through the growth plate and using adipose-derived stem cells (ADSCs) and bone morphogenetic protein-2 (BMP-2) to treat femoral head epiphyseal ischemic necrosis, which can be done in juvenile rabbits. Passagefour bromodeoxyuridine (BrdU)-labeled ADSCs were cultured, assayed with MTT to determine their viability and stained with alizarin red dye to determine their osteogenic ability. Two-month-old, healthy male rabbits (1.2 to 1.4 kg, n=45) underwent ischemic induction and were randomly divided into five groups (group A: animal model control; group B: drilling; group C: drilling & ADSCs; group D: drilling & BMP-2; and group E: drilling & ADSCs & BMP-2). Magnetic resonance imaging (MRI), X-ray imaging, hematoxylin and eosin staining and BrdU immunofluorescence detection were applied 4, 6 and 10 weeks after treatment. Approximately 90% of the ADSCs were labeled with BrdU and showed good viability and osteogenic ability. Similar results were observed in the rabbits in groups C and E at weeks 6 and 10. The animals of groups C and E demonstrated normal hip structure and improved femoral epiphyseal quotients and trabecular areas compared with those of the groups A and B (P |
| Databáze: | OpenAIRE |
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