An effector role for platelets in systemic and local lipopolysaccharide-induced toxicity in mice, mediated by a CD11a- and CD54-dependent interaction with endothelium
| Autor: | J E Ryser, Christian Vesin, Fabienne Tacchini-Cottier, G. Senaldi, Georges E. Grau, Pierre-François Piguet |
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| Rok vydání: | 1993 |
| Předmět: |
Blood Platelets
Lipopolysaccharides Male Pathology medicine.medical_specialty Necrosis medicine.drug_class medicine.medical_treatment Immunology Integrin Galactosamine Pharmacology Biology Monoclonal antibody Microbiology Immunoglobulin G Mice medicine Animals Platelet Mice Inbred BALB C Tumor Necrosis Factor-alpha Cell adhesion molecule Intercellular Adhesion Molecule-1 Lymphocyte Function-Associated Antigen-1 Infectious Diseases Cytokine Mice Inbred CBA biology.protein Female Parasitology Tumor necrosis factor alpha Endothelium Vascular medicine.symptom Cell Adhesion Molecules Research Article |
| Zdroj: | Infection and Immunity. 61:4182-4187 |
| ISSN: | 1098-5522 0019-9567 |
| DOI: | 10.1128/iai.61.10.4182-4187.1993 |
| Popis: | The role of platelets was investigated in two models of lipopolysaccharide (LPS)-induced toxicity in mice: the systemic reaction, provoked by intravenous LPS injection in D-galactosamine-sensitized recipients, which results in host death, and the local reaction, elicited in the skin by sequential injections of LPS and tumor necrosis factor alpha at 24-h intervals, which results in hemorrhagic necrosis. In both models, the depletion of platelets with a rabbit polyclonal or a mouse monoclonal antiplatelet immunoglobulin G afforded significant protection. In the local reaction, studies of the distribution of 111In-labelled platelets as well as optical and electron microscopy showed that platelets are localized in the dermal venules before hemorrhage occurs. Anti-CD11a (LFA-1) and anti-CD54 (ICAM-1) monoclonal antibodies prevented both platelet localization and hemorrhagic necrosis, and these determinants were detected on mouse platelets by immunofluorescence. The antiplatelet monoclonal antibody did not reduce the localization of polymorphonuclear leukocytes in the dermal venules, as shown by histological sections. Thus, in the local reaction, the stimulation with LPS and tumor necrosis factor alpha leads to a binding of platelets to the endothelium of venules by their beta 2 integrins, which seems necessary for the development of the hemorrhagic necrosis. |
| Databáze: | OpenAIRE |
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