Cullin 3SPOP ubiquitin E3 ligase promotes the poly-ubiquitination and degradation of HDAC6
| Autor: | Jinfang Zhang, Brian J. North, Yanpeng Ci, Xiangpeng Dai, Fei Wu, Jianping Guo, Yuyong Tan, Deliang Liu, Jirong Huo |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Proteasome Endopeptidase Complex Ubiquitin-Protein Ligases Amino Acid Motifs SPOP Histone Deacetylase 6 ubiquitination medicine.disease_cause Cell Line 03 medical and health sciences chemistry.chemical_compound Ubiquitin Cell Movement Cullin 3 MG132 medicine Humans Protein Interaction Domains and Motifs Ubiquitins Cell Proliferation Sequence Deletion Genetics biology Protein Stability Autophagosomes Nuclear Proteins HDAC6 Cullin Proteins Ubiquitin ligase Repressor Proteins tumorigenesis 030104 developmental biology Oncology chemistry Proteolysis biology.protein Cancer research Proteasome inhibitor Lysosomes Carcinogenesis Cullin Protein Binding Research Paper medicine.drug |
| Zdroj: | Oncotarget |
| ISSN: | 1949-2553 |
| DOI: | 10.18632/oncotarget.18141 |
| Popis: | // Yuyong Tan 1, 2 , Yanpeng Ci 2, 3 , Xiangpeng Dai 2 , Fei Wu 2, 4 , Jianping Guo 2 , Deliang Liu 1 , Brian J. North 2 , Jirong Huo 1 and Jinfang Zhang 2 1 Department of Gastroenterology, The Second Xiangya Hospital of Central South University, Changsha 410011, P.R. China 2 Department of Pathology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA 02215, USA 3 School of Life Science and Technology, Harbin Institute of Technology, Harbin 150001, P.R. China 4 Department of Urology, Huashan Hospital, Fudan University, Shanghai 200040, P.R. China Correspondence to: Brian J. North, email: bnorth@bidmc.harvard.edu Jirong Huo, email: hjr198@hotmail.com Jinfang Zhang, email: jzhang17@bidmc.harvard.edu Keywords: HDAC6, SPOP, Cullin 3, ubiquitination, tumorigenesis Received: March 29, 2017 Accepted: April 15, 2017 Published: May 24, 2017 ABSTRACT The histone deacetylase 6 (HDAC6) plays critical roles in human tumorigenesis and metastasis. As such, HDAC6-selective inhibitors have entered clinical trials for cancer therapy. However, the upstream regulator(s), especially ubiquitin E3 ligase(s), responsible for controlling the protein stability of HDAC6 remains largely undefined. Here, we report that Cullin 3 SPOP earmarks HDAC6 for poly-ubiquitination and degradation. We found that the proteasome inhibitor MG132, or the Cullin-based E3 ligases inhibitor MLN4924, but not the autophagosome-lysosome inhibitor bafilomycin A1, stabilized endogenous HDAC6 protein in multiple cancer cell lines. Furthermore, we demonstrated that Cullin 3-based ubiquitin E3 ligase(s) primarily reduced the stability of HDAC6. Importantly, we identified SPOP, an adaptor protein of Cullin 3 family E3 ligases, specifically interacted with HDAC6, and promoted its poly-ubiquitination and subsequent degradation in cells. Notably, cancer-derived SPOP mutants disrupted their binding with HDAC6 and thereby failed to promote HDAC6 degradation. More importantly, increased cellular proliferation and migration in SPOP -depleted HCT116 colon cancer cells could be partly reversed by additional depletion of HDAC6 , suggesting that HDAC6 is a key downstream effector for SPOP tumor suppressor function. Together, our data identify the tumor suppressor SPOP as an upstream negative regulator for HDAC6 stability, and SPOP loss-of-function mutations might lead to elevated levels of the HDAC6 oncoprotein to facilitate tumorigenesis and metastasis in various human cancers. |
| Databáze: | OpenAIRE |
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