Platelets are responsible for the accumulation of β-amyloid in blood clots inside and around blood vessels in mouse brain after thrombosis
| Autor: | Lilia Kucheryavykh, Lidia Zueva, A. Valance Washington, David E. Rivera-Aponte, Priscilla Sanabria, Josué Dávila-Rodríguez, Mikhail Inyushin |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Blood Platelets Male Pathology medicine.medical_specialty Neuroscience(all) Immunofluorescence Article law.invention 03 medical and health sciences 0302 clinical medicine In vivo law medicine Animals Entorhinal Cortex Platelet Visual Cortex Amyloid beta-Peptides Microscopy Confocal medicine.diagnostic_test Chemistry Platelet Count General Neuroscience Thrombosis medicine.disease Entorhinal cortex Immunohistochemistry Thrombocytopenia Mice Inbred C57BL Cerebrovascular Disorders Disease Models Animal Microscopy Electron 030104 developmental biology Immunology Female Electron microscope 030217 neurology & neurosurgery Immunostaining Photic Stimulation |
| Zdroj: | Brain research bulletin. 128 |
| ISSN: | 1873-2747 |
| Popis: | Introduction Platelets contain beta-amyloid precursor protein (APP) as well as Aβ peptide (Aβ) that can be released upon activation. During thrombosis, platelets are concentrated in clots and activated. Methods We used in vivo fluorescent analysis and electron microscopy in mice to determine to what degree platelets are concentrated in clots. We used immunostaining to visualize Aβ after photothrombosis in mouse brains. Results Both in vivo results and electron microscopy revealed that platelets were 300–500 times more concentrated in clots than in non-clotted blood. After thrombosis in control mice, but not in thrombocytopenic animals, Aβ immunofluorescence was present inside blood vessels in the visual cortex and around capillaries in the entorhinal cortex. Conclusion The increased concentration of platelets allows enhanced release of Aβ during thrombosis, suggesting an additional source of Aβ in the brains of Alzheimer’s patients that may arise if frequent micro-thrombosis events occur in their brains. |
| Databáze: | OpenAIRE |
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