Ravibacter arvi gen. nov., sp. nov., isolated from farmland soil during development of new culture techniques
| Autor: | Jaisoo Kim, Khulan Altankhuu, Dhiraj Kumar Chaudhary, Ram Hari Dahal |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
DNA Bacterial Farms Lineage (evolution) Cytophagaceae Dyadobacter koreensis medicine.disease_cause Microbiology 03 medical and health sciences Genus RNA Ribosomal 16S Botany Republic of Korea medicine Ecology Evolution Behavior and Systematics Phylogeny Soil Microbiology Base Composition Strain (chemistry) biology Phylogenetic tree Pigmentation Phosphatidylethanolamines Fatty Acids Bacteroidetes Vitamin K 2 General Medicine Sequence Analysis DNA 16S ribosomal RNA biology.organism_classification Bacterial Typing Techniques 030104 developmental biology |
| Zdroj: | International journal of systematic and evolutionary microbiology. 67(12) |
| ISSN: | 1466-5034 |
| Popis: | Two novel strains, J77-1T and J76-1, were isolated from farmland soil and were taxonomically characterized by a polyphasic approach. Both strains were yellow, Gram-stain-negative, strictly aerobic, non-motile and rod-shaped bacteria. These strains were non-sporulating, catalase-positive and oxidase-negative. J77-1T and J76-1 were able to grow at 15–40 °C, pH 5.0–10.0, and 0–1.0 % (w/v) NaCl concentration. Phylogenetic analyses revealed that both strains formed a distinct separate lineage within the family Cytophagaceae of the phylum Bacteroidetes . J77-1T and J76-1 showed low 16S rRNA gene sequence similarity to the most closely related type strain Dyadobacter koreensis WPCB159T (85.09 %) and exhibited less than 85.0 % sequence similarity with other members of the family Cytophagaceae. The pairwise sequence similarity between strains J77-1T and J76-1 was observed to be 99.86 %. In both strains, the only respiratory quinone was menaquinone-7 (MK-7); the major polar lipid was phosphatidylethanolamine; and the major fatty acids were summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c), C16 : 0, iso-C15 : 0, iso-C17 : 0 3-OH C16 : 1ω5c, and C16 : 0 3-OH. The genomic DNA G+C content values of J77-1T and J76-1 were 50.1 and 50.9 mol%, respectively. On the basis of the results of phenotypic, genotypic and phylogenetic analysis, J77-1T represents a novel species of a novel genus, for which the name Ravibacter arvi gen. nov., sp. nov. is proposed, within the family Cytophagaceae. The type strain of Ravibacter arvi is J77-1T (=KEMB 9005-548T=KACC 19172T=JCM 31920T), and J76-1 is an additional strain. |
| Databáze: | OpenAIRE |
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