Lytic Epstein–Barr virus infection in epithelial cells but not in B-lymphocytes is dependent on Blimp1
| Autor: | Hans-Martin Jäck, Gerald Niedobitek, Maike Buettner, Wolfgang Schuh, A. T. Cruchley, Anke Lang, Birgit Meyer, Paul J. Farrell, Christiane S. Tudor, Mário Henrique M. Barros |
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| Rok vydání: | 2012 |
| Předmět: |
B-Lymphocytes
Herpesvirus 4 Human Mononucleosis Cellular differentiation Epithelial Cells Biology Virus Replication medicine.disease Virology Molecular biology Virus Epithelium BZLF1 Repressor Proteins medicine.anatomical_structure Lytic cycle Host-Pathogen Interactions Trans-Activators medicine Humans Luciferase Positive Regulatory Domain I-Binding Factor 1 Epstein–Barr virus infection |
| Zdroj: | Journal of General Virology. 93:1059-1064 |
| ISSN: | 1465-2099 0022-1317 |
| Popis: | Epstein–Barr virus (EBV) replicates in superficial differentiated cells of oral hairy leukoplakia (OHL). Differentiation of squamous epithelial cells depends on B-lymphocyte-induced maturation protein 1 (Blimp1). Here we show that expression of the EBV immediate-early protein BZLF1 is restricted to Blimp1-positive epithelial cells in OHL. Luciferase assays revealed Blimp1-dependent induction of the BZLF1 promoter Zp in epithelial cell lines. Expression of ZEB1, a negative regulator of Zp, and of Xbp-1, which mediates the Blimp1 effect on Zp in B-cells, was not affected by enforced Blimp1 expression. Moreover, Xbp-1 protein expression was not detected in differentiated epithelial cells of OHL. Thus, Blimp1 induces BZLF1 expression in epithelial cells independently of ZEB1 and Xbp-1. In contrast to epithelial cells of OHL, BZLF1 expression was also observed in Blimp1-negative lymphoid cells in infectious mononucleosis tonsils, suggesting that EBV replication in B-cells may be induced independently of terminal differentiation. |
| Databáze: | OpenAIRE |
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