Improved technique for cannulation of the murine thoracic duct: a valuable tool for the dissection of immune responses
Autor: | T. Laskay, Mihai Ionac, G Geisslinger, Werner Solbach, D Labahn |
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Rok vydání: | 1997 |
Předmět: |
medicine.medical_specialty
Microsurgery Lymphocyte medicine.medical_treatment Immunology Thoracic duct Catheterization Thoracic Duct Mice In vivo medicine Immunology and Allergy Animals Fixation (histology) Staining and Labeling business.industry Cannula Surgery Mice Inbred C57BL Dissection medicine.anatomical_structure Anesthesia Female Lymph business |
Zdroj: | Journal of immunological methods. 202(1) |
ISSN: | 0022-1759 |
Popis: | Several experimental approaches have been used in the past for the cannulation of the thoracic duct in mice. Most, however, are characterized by a modest reproducibility and a low lymph yield. Here, we describe a cannulation technique modified with respect to the anesthesia, the use of a silicone cannula and a simple and efficient intraabdominal fixation of the drain. Surgery averaged 45 min with an intra- and postoperative mortality rate of zero. Postoperatively, mice were given access to an exercise wheel allowing increased mobility and consequently a good lymph flow, thus maintaining the function of the cannula. The mice yielded a mean of 29.3 ml/24 h (range 8-40 ml) thoracic duct lymph, which contained a mean of 2.2 x 10(6) lymphocytes/ml during the first 24 h, decreasing to 0.1 x 10(6) lymphocytes/ml on the 2nd day after cannulation. Patency of the cannulae was 100% after 3 days. Interestingly, we have detected strain dependent differences in the anatomy of the thoracic duct in the mouse and these need to be considered when cannulation procedures are attempted. |
Databáze: | OpenAIRE |
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