COVID-19-associated inhibition of energy accumulation pathways in human semen samples
| Autor: | A.A. Stepanian, Y.B. Aznaurova, Alexei Drobyshev, Leila Adamyan, Anton Buzdin, V. I. Vechorko, Dmitriy A. Doroshenko, Maxim Sorokin, Vladimir Elagin, Maria Suntsova, Andrew Garazha, Anton Dashko |
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| Rok vydání: | 2021 |
| Předmět: | |
| Zdroj: | F&s Science |
| ISSN: | 2666-335X |
| Popis: | Objective To investigate transcriptional alterations in human semen samples associated with COVID-19 infection. Design Retrospective observational cohort study. Setting City Hospital. Patients 10 patients who have recovered from mild COVID-19 infection. 8 out of 10 patients had different sperm abnormalities that were diagnosed prior to infection. Control group included 5 healthy donors without known abnormalities and no history of COVID-19. Interventions We used RNA sequencing to determine gene expression profiles in all studied biosamples. Original and standard bioinformatic instruments were used to analyze activation of intracellular molecular pathways. Main Outcome Measures Routine semen analysis, gene expression level and molecular pathway activation levels in semen samples. Results We have revealed statistically significant inhibition of genes associated with energy production pathways in mitochondria, including genes involved in electron transfer chain, and genes involved in Toll-like receptor signaling. In addition, all protein-coding genes encoded by the mitochondrial genome were significantly down-regulated in semen samples collected after recovery from COVID-19. Conclusion Our results may provide molecular basis for the previously observed phenomenon of decreased sperm motility associated with COVID-19 infection. Moreover, identified data would be beneficial for the optimization of preconception care for men who have recently recovered from COVID-19. COVID-19 infection is associated with inhibition of energy accumulation pathways in human semen samples. |
| Databáze: | OpenAIRE |
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