Podocalyxin-like 1 promotes invadopodia formation and metastasis through activation of Rac1/Cdc42/cortactin signaling in breast cancer cells
| Autor: | Mei Ying Liao, Cheng Wei Lin, Kuo Lung Lou, John Yu, Han-Chung Wu, Min Siou Sun, Yi Hsuan Chi, Chu Hung Chung, Li Tin Chiou |
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| Rok vydání: | 2014 |
| Předmět: |
rac1 GTP-Binding Protein
Cancer Research Podosome Sialoglycoproteins RAC1 Breast Neoplasms macromolecular substances Metastasis Focal adhesion medicine Humans Neoplasm Invasiveness Pseudopodia Neoplasm Metastasis cdc42 GTP-Binding Protein Paxillin biology Chemistry General Medicine medicine.disease Extracellular Matrix Gene Expression Regulation Neoplastic Invadopodia Cancer research biology.protein MCF-7 Cells Female Lamellipodium Cortactin Signal Transduction |
| Zdroj: | Carcinogenesis. 35(11) |
| ISSN: | 1460-2180 |
| Popis: | Metastatic disease is the leading cause of cancer mortality. Identifying biomarkers and regulatory mechanisms is important toward developing diagnostic and therapeutic tools against metastatic cancer. In this study, we demonstrated that podocalyxin-like 1 (PODXL) is overexpressed in breast tumor cells and increased in lymph node metastatic cancer. Mechanistically, we found that the expression of PODXL was associated with cell motility and invasiveness. Suppression of PODXL in MDA-MB-231 cells reduced lamellipodia formation and focal adhesion kinase (FAK) and paxillin phosphorylation. PODXL knockdown reduced the formation of invadopodia, such as inhibiting the colocalization of F-actin with cortactin and suppressing phosphorylation of cortactin and neural Wiskott-Aldrich syndrome protein. Conversely, overexpression of PODXL in MCF7 cells induced F-actin/cortactin colocalization and enhanced invadopodia formation and activation. Invadopodia activity and tumor invasion in PODXL-knockdown cells are similar to that in cortactin-knockdown cells. We further found that the DTHL motif in PODXL is crucial for regulating cortactin phosphorylation and Rac1/Cdc42 activation. Inhibition of Rac1/Cdc42 impeded PODXL-mediated cortactin activation and FAK and paxillin phosphorylation. Moreover, inhibition of PODXL in MDA-MB-231 cells significantly suppressed tumor colonization in the lungs and distant metastases, similar to those in cortactin-knockdown cells. These findings show that overexpression of PODXL enhanced invadopodia formation and tumor metastasis by inducing Rac1/Cdc42/cortactin signaling network. |
| Databáze: | OpenAIRE |
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