Characterization of hepatocellular adenoma and carcinoma using microRNA profiling and targeted gene sequencing

Autor: Eran Sadot, T. Peter Kingham, Michael I. D’Angelica, Peter J. Allen, Andrea Ventura, Ronald P. DeMatteo, David S. Klimstra, Jian Zheng, William R. Jarnagin, Joana A. Vigidal, Vinod P. Balachandran
Jazyk: angličtina
Rok vydání: 2018
Předmět:
0301 basic medicine
Male
Viral Diseases
Cirrhosis
Molecular biology
DNA Mutational Analysis
lcsh:Medicine
Pilot Projects
Pathology and Laboratory Medicine
Biochemistry
Hepatitis
Sequencing techniques
Medicine and Health Sciences
Cluster Analysis
lcsh:Science
Immune Response
beta Catenin
Aged
80 and over
Multidisciplinary
Liver Diseases
Liver Neoplasms
RNA sequencing
Genomics
Middle Aged
Adenomas
Immunohistochemistry
3. Good health
Nucleic acids
Gene Expression Regulation
Neoplastic
Infectious Diseases
Oncology
Hepatocellular carcinoma
Female
Research Article
Adult
Carcinoma
Hepatocellular
Adenoma
Immunology
Gastroenterology and Hepatology
Carcinomas
Adenoma
Liver Cell
03 medical and health sciences
Signs and Symptoms
Diagnostic Medicine
microRNA
Gastrointestinal Tumors
medicine
Carcinoma
Genetics
Biomarkers
Tumor
Humans
Non-coding RNA
Aged
Inflammation
Sequence Assembly Tools
Biology and life sciences
business.industry
Sequence Analysis
RNA
lcsh:R
Cancers and Neoplasms
Computational Biology
Hepatocellular Carcinoma
Sequence Analysis
DNA
Hepatocellular adenoma
medicine.disease
Genome Analysis
digestive system diseases
Gene regulation
Research and analysis methods
MicroRNAs
030104 developmental biology
Molecular biology techniques
Cancer research
RNA
lcsh:Q
Gene expression
business
Zdroj: PLoS ONE, Vol 13, Iss 7, p e0200776 (2018)
PLoS ONE
ISSN: 1932-6203
Popis: Background Hepatocellular adenomas (HCA) are benign liver tumors that may transform into hepatocellular carcinoma (HCC), but the molecular drivers of this transformation remain ill-defined. This study evaluates the molecular changes in HCA and HCC and in comparison to their adjacent non-neoplastic liver. Methods 11 patients with HCA and 10 patients with HCC without underlying hepatitis or cirrhosis were included in this pilot study. Tumor and non-tumor liver tissues were selected for immunohistochemical staining, small RNA sequencing, and targeted gene sequencing. We compared microRNA expressions and mutations between HCA and HCC and non-neoplastic liver. Results HCA were classified as inflammatory (n = 6), steatotic (n = 4), or β-catenin activated (n = 1) subtypes. MicroRNA profile of all 3 HCA subtypes clustered between that of normal liver and HCC in principal component analysis. In both HCA and HCC, miR-200a, miR-429, and miR-490-3p were significantly downregulated compared to normal liver, whereas miR-452, miR-766, and miR-1180 were significantly upregulated. In addition, compared to HCA, HCC had significantly higher expression of members of the chromosome 19 miRNA cluster (C19MC), including miR-515-5p, miR-517a, miR-518b, and miR-520c-3p. Conclusions This study indicates that while there are significant differences in the molecular profile between HCA and HCC, several miRNAs are similarly deregulated in HCA and HCC compared to adjacent normal liver. These results may provide insights into the drivers of hepatocarcinogenesis and warrant further investigations.
Databáze: OpenAIRE
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