Intravenous injection of urocortin 1 induces a CRF2 mediated increase in circulating ghrelin and glucose levels through distinct mechanisms in rats
| Autor: | Andreas Stengel, Pu-Qing Yuan, Lixin Wang, Yvette Taché, Almaas Shaikh, Miriam Goebel-Stengel |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Blood Glucose
Male Agonist endocrine system medicine.medical_specialty Corticotropin-Releasing Hormone Physiology medicine.drug_class Autonomic Nervous System Peptides Cyclic Receptors Corticotropin-Releasing Hormone Biochemistry Article Rats Sprague-Dawley Cellular and Molecular Neuroscience chemistry.chemical_compound Corticotropin-releasing hormone Endocrinology Internal medicine Orexigenic otorhinolaryngologic diseases medicine Animals Humans Urocortins Urocortin Gastric emptying digestive oral and skin physiology Ghrelin Peptide Fragments Rats chemistry Injections Intravenous Hexamethonium hormones hormone substitutes and hormone antagonists medicine.drug |
| Zdroj: | Peptides. 39:164-170 |
| ISSN: | 0196-9781 |
| DOI: | 10.1016/j.peptides.2012.11.009 |
| Popis: | Urocortins (Ucns) injected peripherally decrease food intake and gastric emptying through peripheral CRF(2) receptors in rodents. However, whether Ucns influence circulating levels of the orexigenic and prokinetic hormone, ghrelin has been little investigated. We examined plasma levels of ghrelin and blood glucose after intravenous (iv) injection of Ucn 1, the CRF receptor subtype involved and underlying mechanisms in ad libitum fed rats equipped with a chronic iv cannula. Ucn 1 (10 μg/kg, iv) induced a rapid onset and long lasting increase in ghrelin levels reaching 68% and 219% at 0.5 and 3h post injection respectively and a 5-h hyperglycemic response. The selective CRF(2) agonist, Ucn 2 (3 μg/kg, iv) increased fasting acyl (3h: 49%) and des-acyl ghrelin levels (3h: 30%) compared to vehicle while the preferential CRF(1) agonist, CRF (3 μg/kg, iv) had no effect. Ucn 1's stimulatory actions were blocked by the selective CRF(2) antagonist, astressin(2)-B (100 μg/kg, iv). Hexamethonium (10 mg/kg, sc) prevented Ucn 1-induced rise in total ghrelin levels while not altering the hyperglycemic response. These data indicate that systemic injection of Ucns induces a CRF(2)-mediated increase in circulating ghrelin levels likely via indirect actions on gastric ghrelin cells that involves a nicotinic pathway independently from the hyperglycemic response. |
| Databáze: | OpenAIRE |
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