Regulation of the interleukin-5 receptorα-subunit on peripheral blood eosinophils from healthy subjects
| Autor: | Britta Hylander, G Halldén, Joachim Lundahl, C Hellman |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Male
medicine.medical_specialty medicine.medical_treatment Immunology Alpha (ethology) Biology Internal medicine Eosinophilia medicine Ascitic Fluid Humans Immunology and Allergy Autocrine signalling Interleukin 5 Cells Cultured Aged Interleukin-5 receptor Antibodies Monoclonal Granulocyte-Macrophage Colony-Stimulating Factor Original Articles Middle Aged Eosinophil Recombinant Proteins Eosinophils Protein Subunits Cytokine Endocrinology medicine.anatomical_structure Depression Chemical Female Interleukin-5 medicine.symptom Alpha chain |
| Zdroj: | Clinical and Experimental Immunology. 131:75-81 |
| ISSN: | 1365-2249 0009-9104 |
| DOI: | 10.1046/j.1365-2249.2003.02051.x |
| Popis: | SUMMARYThe aim was to study in vitro regulation of the IL-5 receptor α (IL-5Rα) on purified peripheral blood eosinophils from healthy subjects. The IL-5Rα was down-regulated, in a dose-dependent manner, by recombinant IL-5 and GM-CSF, with IL-5 being most potent. This down-regulation was not induced by autocrine release of GM-CSF or IL-5, respectively. Incubation of eosinophils with cell-free peritoneal dialysis fluid (PF) collected from a patient with peritoneal fluid eosinophilia (PFE), induced up-regulation of the proportion of CD69 positive eosinophils, in parallel with down-regulation of the proportion of IL-5Rα positive eosinophils. Experiments with neutralizing antibodies against IL-5 and GM-CSF, revealed that IL-5 was the principal cytokine responsible for the down-regulation of the IL-5Rα. When eosinophils were incubated with PF collected from the same patient in remission or with PF collected from a newly started patient or a patient with bacterial peritonitis, less down-regulation of the IL-5Rα was observed.In conclusion our data indicate that IL-5, as opposed to its proposed action on eosinophil progenitors, down-regulates the IL-5Rα chain on mature eosinophils. We therefore suggest that an IL-5 driven inflammation generates an eosinophil tissue phenotype that is characterized by a low IL-5Rα expression. These aspects of IL-5 action on IL-5Rα expression could gain new insights into the mechanisms of specific immuno-modulatory therapies, such as anti-IL-5. |
| Databáze: | OpenAIRE |
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