Kinetic analysis of the antibacterial activity of probiotic lactobacilli towards Salmonella enterica serovar Typhimurium reveals a role for lactic acid and other inhibitory compounds

Autor: Georgia Zoumpopoulou, Domitille Fayol-Messaoudi, Vagelis Triantafyllou, Effie Tsakalidou, Luc De Vuyst, Alain L. Servin, Tom Adriany, Lefteris Makras
Přispěvatelé: Resarch Group of Industrial Microbiology, Vrije Universiteit Brussel (VUB), Laboratory of dairy research, Agricultural University of Athens, Pathogenes et Fonctions des Cellules Epitheliales Polarisees, Université Paris-Sud - Paris 11 (UP11)-Institut National de la Santé et de la Recherche Médicale (INSERM), Codogno, Patrice
Rok vydání: 2005
Předmět:
Salmonella typhimurium
Salmonella
MESH: Hydrogen-Ion Concentration
medicine.disease_cause
Microbiology
MESH: Fermentation
law.invention
03 medical and health sciences
chemistry.chemical_compound
Probiotic
fluids and secretions
law
[SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases
Lactobacillus
medicine
Humans
Lactic Acid
[SDV.MP] Life Sciences [q-bio]/Microbiology and Parasitology
Molecular Biology
030304 developmental biology
MESH: Microbial Viability
0303 health sciences
MESH: Humans
Microbial Viability
biology
030306 microbiology
Probiotics
food and beverages
MESH: Salmonella typhimurium
General Medicine
Lactobacillaceae
Hydrogen-Ion Concentration
biology.organism_classification
Lactic acid
[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology
chemistry
Salmonella enterica
Fermentation
[SDV.MHEP.MI] Life Sciences [q-bio]/Human health and pathology/Infectious diseases
MESH: Lactic Acid
MESH: Caco-2 Cells
Caco-2 Cells
Antibacterial activity
MESH: Lactobacillus
Bacteria
MESH: Probiotics
Zdroj: Research in Microbiology
Research in Microbiology, Elsevier, 2006, 157 (3), pp.241-7. ⟨10.1016/j.resmic.2005.09.002⟩
ISSN: 0923-2508
DOI: 10.1016/j.resmic.2005.09.002⟩
Popis: Six Lactobacillus strains including commercial probiotic ones (L. acidophilus IBB 801, L. amylovorus DCE 471, L. casei Shirota, L. johnsonii La1, L. plantarum ACA-DC 287 and L. rhamnosus GG) were investigated, through batch fermentations under controlled conditions, for their capacity to inhibit Salmonella enterica serovar Typhimurium SL1344. All lactobacilli displayed strong antibacterial activity toward this Gram-negative pathogen and significantly inhibited invasion of the pathogen into cultured human enterocyte-like Caco-2/TC7 cells. By studying the production kinetics of antibacterial activity and applying the appropriate acid and pH control samples during a killing assay, we were able to distinguish between the effect of lactic acid and other inhibitory compounds produced. The antibacterial activity of L. acidophilus IBB 801, L. amylovorus DCE 471, L. casei Shirota and L. rhamnosus GG was solely due to the production of lactic acid. The antibacterial activity of L. johnsonii La1 and L. plantarum ACA-DC 287 was due to the production of lactic acid and (an) unknown inhibitory substance(s). The latter was (were) only active in the presence of lactic acid. In addition, the lactic acid produced was responsible for significant inhibitory activity upon invasion of Salmonella into Caco-2/TC7 cells.
Databáze: OpenAIRE
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