A Three-Dimensional Model of a Group II Intron RNA and Its Interaction with the Intron-Encoded Reverse Transcriptase
| Autor: | Dinggeng Chai, Alan M. Lambowitz, Steven Zimmerly, Forrest Blocker, Lixin Dai, Jesse Gabel, Shan Qing Gu, Sergei Y. Noskov |
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| Rok vydání: | 2008 |
| Předmět: |
Models
Molecular Spliceosome Protein Conformation RNA Splicing Molecular Sequence Data Biology Article 03 medical and health sciences Bacterial Proteins RNA Catalytic Group I catalytic intron Amino Acid Sequence Molecular Biology Phylogeny 030304 developmental biology Genetics 0303 health sciences Binding Sites 030302 biochemistry & molecular biology Intron Ribozyme RNA RNA-Directed DNA Polymerase Cell Biology Group II intron Circular permutation in proteins Introns Cross-Linking Reagents RNA splicing biology.protein Nucleic Acid Conformation |
| Zdroj: | Molecular Cell. 30:472-485 |
| ISSN: | 1097-2765 |
| DOI: | 10.1016/j.molcel.2008.04.001 |
| Popis: | Group II introns are self-splicing ribozymes believed to be the ancestors of spliceosomal introns. Many group II introns encode reverse transcriptases that promote both RNA splicing and intron mobility to new genomic sites. Here we used a circular permutation and cross-linking method to establish sixteen intramolecular distance relationships within the mobile Lactococcus lactis Ll.LtrB-ΔORF intron. Using these new constraints together with thirteen established tertiary interactions and eight published cross-links, we modeled a complete three-dimensional structure of the intron. We also used the circular permutation strategy to map RNA-protein interaction sites through fluorescence quenching and cross-linking assays. Our model provides a comprehensive structural framework for understanding the function of group II ribozymes, their natural structural variations, and the mechanisms by which the intron-encoded protein promotes RNA splicing and intron mobility. The model also suggests an arrangement of active site elements that may be conserved in the spliceosome. |
| Databáze: | OpenAIRE |
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