Rapid diagnosis of pulmonary tuberculosis by using Roche AMPLICOR Mycobacterium tuberculosis PCR test
| Autor: | M Ghouri, R F D'Amato, A A Wallman, Kyungmee Kim, M Scardamaglia, Albert Miller, E Ardila, Peter M. Colaninno, Rajesh Patel, L Hochstein |
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| Rok vydání: | 1995 |
| Předmět: |
Microbiology (medical)
Tuberculosis Time Factors Polymerase Chain Reaction Mycobacterium tuberculosis Pulmonary tuberculosis Pcr test medicine Humans Diagnostic Errors Tuberculosis Pulmonary Bacteriological Techniques biology business.industry virus diseases medicine.disease biology.organism_classification equipment and supplies bacterial infections and mycoses Virology Predictive value Lung disease Evaluation Studies as Topic Clinical diagnosis Immunology business Research Article |
| Zdroj: | Journal of clinical microbiology. 33(7) |
| ISSN: | 0095-1137 |
| Popis: | A rapid PCR-based test for the diagnosis of pulmonary tuberculosis, the Roche AMPLICOR Mycobacterium tuberculosis test (AMPLICOR MTB), was evaluated. Results from AMPLICOR MTB were compared with culture results and the final clinical diagnosis for each patient. A total of 985 specimens from 372 patients were tested. When AMPLICOR MTB results were compared with resolved results, i.e., a specimen grew M. tuberculosis or was obtained from a patient with a clinical diagnosis of tuberculosis, the sensitivity, specificity, positive predictive value, and negative predictive value for the AMPLICOR MTB test were 66.7, 99.6, 91.7, and 97.7%, respectively. These results were comparable to those obtained from culture. Test results were available approximately 6.5 h after specimen receipt in the laboratory. Our data demonstrate that AMPLICOR MTB will provide rapid, valuable information for the diagnosis and control of tuberculosis. |
| Databáze: | OpenAIRE |
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