Semen cryopreservation of small abalone (Haliotis diversicolor supertexa)
| Autor: | Jin-Chywan Gwo, Hsien-Yu Cheng, Chuan-Wen Chen |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Male
Time Factors Cryoprotectant Taiwan Semen Aquaculture Biology Cryopreservation law.invention chemistry.chemical_compound Cryoprotective Agents Food Animals law Botany Animals Small Animals Sperm motility Sperm-Ovum Interactions Chromatography urogenital system Equine Dimethyl sulfoxide Extender Age Factors Semen cryopreservation Sperm Spermatozoa Fertility chemistry Mollusca Sperm Motility Animal Science and Zoology Female Semen Preservation |
| Zdroj: | Theriogenology. 58(8) |
| ISSN: | 0093-691X |
| Popis: | Methods for cryopreserving spermatozoa and maximizing fertilization rate in Taiwan small abalone, Haliotis diversicolor supertexa, were developed. The gametes (spermatozoa and eggs) of small abalone were viable 3 h post-spawning, with fertilization, and development rate decreasing with time. A minimum of 10(2) cell/ml sperm concentration and a contact time of 2 min between gametes is recommended for artificial insemination of small abalone eggs. Eight cryoprotectants, dimethyl sulfoxide (DMSO), dimethyl acetamide (DMA), ethylene glycol (EG), propylene glycol (PG), butylene glycol (BG), polyethylene glycol, glycerol and methanol, were tested at concentrations between 5 and 25% to evaluate their effect on motility of spermatozoa exposed to cryoprotectant for up to 60 min at 25 degrees C before freezing. The least toxic cryoprotectant, 10% DMSO, was added to artificial seawater (ASW) to formulate the extender for freezing. Semen was diluted 1:1 with the extender, inserted into 1.5 ml microtubes and frozen using a cooling rate between -3.5 and -20 degrees C/min to various transition temperatures (0, -30, -60, -90 and -120 degrees C), followed by transfer and storage in liquid nitrogen (-196 degrees C). The microtubes were thawed from +45 to +145 degrees C/min. Spermatozoa, cooled to -90 degrees C at a cooling rate of -12 or -15 degrees C/min and then immersed in liquid nitrogen, had the best post-thaw motility. Post-thaw sperm motility was markedly reduced compared to fresh sperm. More frozen-thawed spermatozoa are required to achieve fertilization rates comparable to those achieved using fresh spermatozoa. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect