Detection of Different Serotypes of Salmonella enterica in Experimentally Inoculated Equine Fecal Samples by Commercially Available Rapid Tests
| Autor: | Brandy A. Burgess, D.C. Van Metre, Paul S. Morley, Denise Bolte, Kristy L. Pabilonia, C.B. Weller |
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| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Serotype
Salmonella Point-of-Care Systems dnaH Culture Standard Article medicine.disease_cause Horse Real-Time Polymerase Chain Reaction Microbiology Feces Lateral flow immunoassay medicine Animals Horses Serotyping Colony-forming unit Immunoassay Bacteriological Techniques Salmonella Infections Animal General Veterinary biology Inoculation DNA–DNA hybridization Nucleic Acid Hybridization Salmonella enterica biology.organism_classification Virology Standard Articles Horse Diseases Reagent Kits Diagnostic |
| Zdroj: | Journal of Veterinary Internal Medicine |
| ISSN: | 1939-1676 0891-6640 |
| Popis: | Background Salmonella enterica can significantly impact management of animal facilities. Comprehensive screening is essential for effective control in high-risk populations. Availability of reliable point-of-care diagnostic tests would facilitate these efforts. Hypothesis/Objectives Compare the ability of commercially available rapid diagnostic assays (2 lateral flow immunoassays [LFIs], DNA hybridization [DNAH], real-time PCR [qPCR]), and culture to detect common serotypes of S. enterica in feces. Animals n/a. Methods In an experimental study, 112 S. enterica isolates were randomly selected from the 10 most common serotypes recovered at a veterinary hospital. Archived isolates were amplified in broth and standardized inocula (100 colony forming units) were incubated with equine feces in tetrathionate broth (TET). Cultures were tested in a blinded fashion by using LFIs, DNAH, qPCR, and culture. Results The LFIs detected 84% and 67% of isolates, respectively, but reactivity varied among serotypes. Both reacted poorly with serotype Cerro (Group K) isolates, and 1 LFI did not react with any serotype Mbandaka (Group C1) or Montevideo (Group C1) isolates. DNAH detected 94% of isolates, whereas culture and qPCR most reliably detected all serotypes. False-positive results were obtained for 4 negative controls by using DNAH and 1 negative control by using qPCR, but LFIs and culture had no false-positive results. Conclusions and Clinical Importance Culture, qPCR, and DNAH were effective in detecting most Salmonella isolates, but have limited application at point-of-care settings. LFIs are appealing as point-of-care tests because of low cost and ease of use, but limited detection of some serotypes needs to be evaluated with samples obtained from naturally infected animals. |
| Databáze: | OpenAIRE |
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