Separation of racemic 2,4-dinitrophenyl amino acids on zirconia-immobilized quinine carbamate in reversed-phase liquid chromatography
| Autor: | In Whan Kim, Jung Hag Park, Wonjae Lee, Joon Woo Lee, Jae Jeong Ryoo, Chun Sup Ra, Jin Soon Cha, Young Tae Song, Myung Duk Jang |
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| Rok vydání: | 2004 |
| Předmět: |
Silicon
Carbamate Time Factors medicine.medical_treatment Filtration and Separation Analytical Chemistry Phase (matter) medicine Organic chemistry Cubic zirconia Lewis acids and bases Amino Acids chemistry.chemical_classification Chromatography Quinine Chemistry Temperature Stereoisomerism Reversed-phase chromatography Hydrogen-Ion Concentration Amino acid Dinitrobenzenes Carbamates Zirconium Enantiomer Selectivity Chromatography Liquid |
| Zdroj: | Journal of Separation Science. 27:977-982 |
| ISSN: | 1615-9314 1615-9306 |
| DOI: | 10.1002/jssc.200301713 |
| Popis: | Zirconia is known to be one of the best chromatographic support materials due to its excellent chemical, thermal, and mechanical stability. A quinine carbamate-coated zirconia was prepared as a chiral stationary phase for separation of enantiomers of DNP-amino acids in reversed-phase liquid chromatography. Retention and enantioselectivity of this phase were compared to those for quinine carbamate bonded onto silica. Most amino acids studied were separated on the quinine carbamate-zirconia CSP although retention was longer and chiral selectivity was somewhat lower than on the corresponding silica CSP. Increased retention and decreased selectivity are probably due to strong non-enantioselective Lewis acid-base interactions between the amino acid molecule and the residual Lewis acid sites on the zirconia surface. |
| Databáze: | OpenAIRE |
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