Hydrosalpingeal fluid inhibits in-vitro embryonic development in a murine model
| Autor: | Stan A. Beyler, William R. Meyer, Marc A. Fritz, Kay P. James |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
medicine.medical_specialty
animal structures Uterus Fertilization in Vitro Biology Andrology Embryonic and Fetal Development Mice Human fertilization Culture Techniques medicine Animals Humans Blastocyst Fallopian Tubes Hydrosalpinx Gynecology Rehabilitation Embryogenesis Proteins Obstetrics and Gynecology Embryo culture Embryo Fallopian Tube Diseases Embryo Transfer medicine.disease Embryo transfer Body Fluids Culture Media Glucose medicine.anatomical_structure Reproductive Medicine embryonic structures Female Infertility Female |
| Zdroj: | Human Reproduction. 12:2724-2728 |
| ISSN: | 1460-2350 0268-1161 |
| DOI: | 10.1093/humrep/12.12.2724 |
| Popis: | Recent evidence describing a suboptimal clinical outcome in women with hydrosalpinges who undergo in-vitro fertilization (IVF) and embryo transfer suggests a potential deleterious effect of this fluid on in-utero embryo development. Consequently, we evaluated in-vitro mouse embryo development in the presence of hydrosalpingeal fluid (HF) collected from 10 infertile women of reproductive age. Chemical analyses showed both similarities and differences of these fluids to reported values for fluids collected from non-diseased Fallopian tubes. The HF had a significant deleterious effect upon mouse embryo cleavage and development to the expanded and hatched blastocyst stage, although the effect was variable among patients. Dilution of HF to 30% concentration with culture medium failed to negate this effect. This argues against the effect resulting from a relative lack of critical, supportive component(s) in the HF. Additionally, further experiments performed with cultures under an oil overlay significantly reduced the embryotoxicity of the HF. This evidence suggests there may be a lipophilic factor that can impair embryo development. The relatively poor IVF-embryo transfer success in women with proximally patent hydrosalpinges may be explained, at least in part, by reflux of a lipophilic embryotoxic factor(s) into the uterine cavity. |
| Databáze: | OpenAIRE |
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